Le Gall Hyacinthe, Fontaine Jean-Xavier, Molinié Roland, Pelloux Jérôme, Mesnard François, Gillet Françoise, Fliniaux Ophélie
EA3900-BIOPI, Biologie des Plantes et Innovation, Université de Picardie Jules Verne, 80039, Amiens, France.
Phytochem Anal. 2017 Jan;28(1):58-67. doi: 10.1002/pca.2649.
Abiotic stress is a major cause of yield loss in plant culture. Miscanthus, a perennial C4 grass, is now considered a major source of renewable energy, especially for biofuel production. During the first year of planting in Northern Europe, Miscanthus was exposed to frost temperature, which generated high mortality in young plants and large loss of yield. One strategy to avoid such loss is to apply cold-acclimation, which confers on plants a better resistance to low temperature.
The aim of this study is to describe the effect of a cold-acclimation period on the metabolome of two Miscanthus genotypes that vary in their frost sensitivity at the juvenile stage. Miscanthus × giganteus (GIG) is particularly sensitive to frost, whereas Miscanthus sinensis August Feder (AUG) is tolerant.
Polar metabolite extraction was performed on Miscanthus, grown in non-acclimated or cold-acclimated conditions. Extracts were analysed by H-NMR followed by multivariate statistical analysis. Discriminant metabolites were identified.
More than 40 metabolites were identified in the two Miscanthus genotypes. GIG and AUG showed a different metabolic background before cold treatment, probably related to their genetic background. After cold-acclimation, GIG and AUG metabolomes remained different. The tolerant genotype showed notably higher levels of accumulation in proline, sucrose and maltose when subjected to cold.
These two genotypes seem to have a different adaptation strategy in cold conditions. The studied change in the metabolome concerns different types of molecules related to the cold-tolerant behaviour of Miscanthus. Copyright © 2016 John Wiley & Sons, Ltd.
非生物胁迫是植物栽培中产量损失的主要原因。芒草是一种多年生C4禾本科植物，现在被认为是可再生能源的主要来源，特别是用于生物燃料生产。在北欧种植的第一年，芒草遭遇了霜冻温度，导致幼苗死亡率很高，产量大幅损失。避免这种损失的一种策略是进行冷驯化，这能使植物对低温具有更好的抗性。
本研究的目的是描述冷驯化期对两种芒草基因型代谢组的影响，这两种基因型在幼苗期对霜冻的敏感性不同。巨芒（GIG）对霜冻特别敏感，而八月芒（AUG）具有耐受性。
对在未驯化或冷驯化条件下生长的芒草进行极性代谢物提取。提取物通过氢核磁共振分析，随后进行多变量统计分析。鉴定出具有鉴别作用的代谢物。
在两种芒草基因型中鉴定出40多种代谢物。GIG和AUG在冷处理前显示出不同的代谢背景，这可能与其遗传背景有关。冷驯化后，GIG和AUG的代谢组仍然不同。在遭受低温时，耐受性基因型的脯氨酸、蔗糖和麦芽糖积累水平明显更高。
这两种基因型在寒冷条件下似乎有不同的适应策略。代谢组的研究变化涉及与芒草耐寒行为相关的不同类型分子。版权所有©2016约翰威立父子有限公司。
