Bioimaging Core, Faculty of Health Sciences, University of Macau, Macau SAR, 999078, P. R. China.
Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, P. R. China.
Adv Healthc Mater. 2017 Jan;6(1). doi: 10.1002/adhm.201601094. Epub 2016 Dec 15.
Controllable preparation of water-soluble multifunctional nanoprobes is of great significance for cancer early diagnosis. In this study, protein-modified hydrophilic copper sufide (CuS) nanotriangles with tunable absorption in the second near-infrared region are developed in the presence of halide ions. Further, gadolinium ions chelated diethylenetriaminepentaacetic acid is conjugated on it by using the unique characteristics of the protein-protected nanotriangles. Specifically, the as-obtained nanostructures are investigated as contrast agents for enhanced in vivo photoacoustic/magnetic resonance dual-modal tumor imaging. More importantly, in vitro and in vivo toxicity analysis are also performed, which show that the dual-modal nanoprobes are biocompatible for most of the cases. It is demonstrated that the novel as-prepared protein-modified nanotriangles are able to work as a nanoplatform to construct dual-modal nanoprobes, which paves a new avenue for improving the photoacoustic/magnetic resonance imaging contrast in cancer detection. It should be pointed out that other functional blocks may also be linked on it, which makes it a general method to design multifunctional nanoprobes.
可控制备水溶性多功能纳米探针对于癌症的早期诊断具有重要意义。在这项研究中，在卤化物离子存在的情况下，制备了具有可调谐近红外二区吸收的蛋白修饰亲水性硫化铜纳米三角体。此外，利用蛋白保护纳米三角体的独特性质，将螯合二乙烯三胺五乙酸的钆离子进一步连接在其上。具体来说，将所获得的纳米结构作为对比剂，用于增强体内光声/磁共振双模肿瘤成像。更重要的是，还进行了体外和体内毒性分析，结果表明大多数情况下，双模态纳米探针具有良好的生物相容性。实验证明，新型制备的蛋白修饰纳米三角体可用作构建双模态纳米探针的纳米平台，为提高癌症检测中的光声/磁共振成像对比度开辟了新途径。应该指出的是，其他功能模块也可以与之相连，这使得它成为设计多功能纳米探针的通用方法。
