Section of Orofacial Pain and Jaw Function, Department of Dental Medicine, Karolinska Institutet, and Scandinavian Center for Orofacial Neurosciences (SCON), SE 14104 Huddinge, Sweden.
Folktandvården Stockholm AB, Sweden.
Sci Rep. 2016 Dec 15;6:39073. doi: 10.1038/srep39073.
Determination of the variability in the salivary proteome is a prerequisite for the development of saliva as a diagnostic and prognostic tool in particular physiological states. In this context, it is important that technical variability induced by sample collection and processing is kept at minimum to be able to reproducibly assess variability in states of health and disease. In the current study, the proteome profile in unstimulated and stimulated whole, parotid and sublingual saliva was investigated using two-dimensional gel electrophoresis. Saliva samples were structurally collected from ten examined and characterized healthy individuals during the exactly same conditions. The results demonstrated that different collection methods provide clear differences in the snapshot of the salivary proteome and also in the relative amount of specific proteins. The variable nature of the salivary proteome suggests that different approaches may have to be adopted when studying its composition or its possible role as an indicator for particular physiological states. The results emphasize the importance of consistency when collecting saliva samples for proteomic analysis.
确定唾液蛋白质组的变异性是将唾液发展为特定生理状态下的诊断和预后工具的前提。在这种情况下，通过样本收集和处理引起的技术变异性保持在最低水平非常重要，以便能够重复评估健康和疾病状态下的变异性。在本研究中，使用二维凝胶电泳技术研究了未刺激和刺激的全唾液、腮腺和舌下唾液中的蛋白质组图谱。在完全相同的条件下，从十位经检查和特征分析的健康个体中结构性收集唾液样本。结果表明，不同的收集方法在唾液蛋白质组的快照和特定蛋白质的相对含量方面提供了明显的差异。唾液蛋白质组的可变性表明，在研究其组成或其作为特定生理状态指标的可能作用时，可能需要采用不同的方法。研究结果强调了在进行蛋白质组分析时收集唾液样本时保持一致性的重要性。
