Ahijado-Guzmán Rubén, Menten Julia, Prasad Janak, Lambertz Christina, Rivas Germán, Sönnichsen Carsten
Institute of Physical Chemistry, University of Mainz , Duesbergweg 10-14, D-55128 Mainz, Germany.
Graduate School Materials Science in Mainz , Staudingerweg 9, D-55128 Mainz, Germany.
ACS Appl Mater Interfaces. 2017 Jan 11;9(1):218-223. doi: 10.1021/acsami.6b14013. Epub 2016 Dec 22.
We demonstrate the potential of the NanoSPR (nanoscale surface plasmon resonance sensors) method as a simple and cheap tool for the quantitative study of membrane protein-protein interactions. We use NanoSPR to determine the effectiveness of two potential drug candidates that inhibit the protein complex formation between FtsA and ZipA at initial stages of bacterial division. As the NanoSPR method relies on individual gold nanorods as sensing elements, there is no need for fluorescent labels or organic cosolvents, and it provides intrinsically high statistics. NanoSPR could become a powerful tool in drug development, drug delivery, and membrane studies.
我们展示了纳米表面等离子体共振传感器（NanoSPR）方法作为一种用于定量研究膜蛋白-蛋白相互作用的简单且廉价工具的潜力。我们使用NanoSPR来确定两种潜在药物候选物在细菌分裂初始阶段抑制FtsA和ZipA之间蛋白质复合物形成的有效性。由于NanoSPR方法依赖于单个金纳米棒作为传感元件，因此无需荧光标记或有机助溶剂，并且其本身提供了高统计量。NanoSPR可能会成为药物开发、药物递送和膜研究中的强大工具。
