Institute of Physical Chemistry, University of Mainz , Duesbergweg 10-14, D-55128 Mainz, Germany.
Nano Lett. 2014 Oct 8;14(10):5528-32. doi: 10.1021/nl501865p. Epub 2014 Sep 8.
Most of current techniques used for the quantification of protein-protein interactions require the analysis of one pair of binding partners at a time. Herein we present a label-free, simple, fast, and cost-effective route to characterize binding affinities between multiple macromolecular partners simultaneously, using optical dark-field spectroscopy and individual protein-functionalized gold nanorods as sensing elements. Our NanoSPR method could easily become a simple and standard tool in biological, biochemical, and medical laboratories.
目前大多数用于定量蛋白质-蛋白质相互作用的技术一次只能分析一对结合配偶体。在此，我们提出了一种无标记、简单、快速且经济有效的方法，使用光学暗场光谱和单个蛋白功能化的金纳米棒作为传感元件，同时对多个大分子配体之间的结合亲和力进行表征。我们的 NanoSPR 方法可以很容易地成为生物、生化和医学实验室的简单标准工具。
