Luo Xie, Wang Han, Leighton Jake, O'Sullivan Mara, Wang Pei
Department of Cellular & Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA.
Regen Med. 2017 Jan;12(1):77-89. doi: 10.2217/rme-2016-0086. Epub 2016 Dec 15.
Definitive endoderm is the cellular precursor to respiratory- and digestive-related organs such as lungs, stomach, liver, pancreas and intestine. Endodermal lineage cells derived from pluripotent stem cells (PSCs) in vitro are a potentially unlimited resource for regenerative medicine. These cells are useful tools for studying the physiology, pathogenesis and medical therapies involving these tissues, and great progress has been achieved in PSCs differentiation protocols. In this review, we will focus on the most common and/or advanced differentiation strategies currently used in generating endodermal lineage cells from PSCs. A brief discussion about the effect of early definitive endoderm differentiation on the final development products will follow.
确定内胚层是肺、胃、肝脏、胰腺和肠道等呼吸及消化相关器官的细胞前体。体外源自多能干细胞（PSC）的内胚层谱系细胞是再生医学中潜在的无限资源。这些细胞是研究涉及这些组织的生理学、发病机制和医学疗法的有用工具，并且在PSC分化方案方面已经取得了巨大进展。在本综述中，我们将重点关注目前用于从PSC生成内胚层谱系细胞的最常见和/或先进的分化策略。随后将简要讨论早期确定内胚层分化对最终发育产物的影响。
