Tokunaga Eriko, Yamashita Nami, Tanaka Kimihiro, Inoue Yuka, Akiyoshi Sayuri, Saeki Hiroshi, Oki Eiji, Kitao Hiroyuki, Maehara Yoshihiko
Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Maidashi, Higashi-ku, Fukuoka, Japan.
Department of Molecular Oncology, Graduate School of Medical Sciences, Kyushu University, Maidashi, Higashi-ku, Fukuoka, Japan.
PLoS One. 2016 Dec 15;11(12):e0168090. doi: 10.1371/journal.pone.0168090. eCollection 2016.
Recent studies have identified the apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3B (APOBEC3B) as a source of mutations in various malignancies. APOBEC3B is overexpressed in several human cancer types, including breast cancer. In this study, we analyzed APOBEC3B mRNA expression in 305 primary breast cancers of Japanese women using quantitative reverse transcription-PCR, and investigated the relationships between the APOBEC3B mRNA expression and clinicopathological characteristics, prognosis, and TP53 mutations. The expression of APOBEC3B mRNA was detected in 277 tumors and not detected in 28 tumors. High APOBEC3B mRNA expression was significantly correlated with ER- and PR-negativity, high grade and high Ki67 index. The APOBEC3B mRNA expression was highest in the triple-negative and lowest in the hormone receptor-positive/HER2-negative subtypes. The TP53 gene was more frequently mutated in the tumors with high APOBEC3B mRNA expression. High APOBEC3B mRNA expression was significantly associated with poor recurrence-free survival in all cases and the ER-positive cases. These findings were almost consistent with the previous reports from the Western countries. In conclusion, high APOBEC3B mRNA expression was related to the aggressive phenotypes of breast cancer, high frequency of TP53 mutation and poor prognosis, especially in ER-positive tumors.
最近的研究已确定载脂蛋白B信使核糖核酸编辑酶催化多肽样3B（APOBEC3B）是各种恶性肿瘤中突变的一个来源。APOBEC3B在包括乳腺癌在内的几种人类癌症类型中过表达。在本研究中，我们使用定量逆转录聚合酶链反应分析了305例日本女性原发性乳腺癌中APOBEC3B信使核糖核酸的表达，并研究了APOBEC3B信使核糖核酸表达与临床病理特征、预后及TP53突变之间的关系。在277个肿瘤中检测到APOBEC3B信使核糖核酸的表达，在28个肿瘤中未检测到。APOBEC3B信使核糖核酸的高表达与雌激素受体和孕激素受体阴性、高级别及高Ki67指数显著相关。APOBEC3B信使核糖核酸表达在三阴性亚型中最高，在激素受体阳性/人表皮生长因子受体2阴性亚型中最低。在APOBEC3B信使核糖核酸高表达的肿瘤中，TP53基因更频繁地发生突变。APOBEC3B信使核糖核酸高表达在所有病例及雌激素受体阳性病例中均与无复发生存期差显著相关。这些发现与西方国家先前的报道几乎一致。总之，APOBEC3B信使核糖核酸高表达与乳腺癌的侵袭性表型、TP53突变的高频率及不良预后相关，尤其是在雌激素受体阳性肿瘤中。
