脉冲电场对酿酒酵母细胞中硒和锌离子积累的影响。
Effect of pulsed electric fields (PEF) on accumulation of selenium and zinc ions in Saccharomyces cerevisiae cells.
作者信息
Pankiewicz Urszula, Sujka Monika, Kowalski Radosław, Mazurek Artur, Włodarczyk-Stasiak Marzena, Jamroz Jerzy
机构信息
Department of Analysis and Evaluation of Food Quality, Faculty of Food Science and Biotechnology, University of Life Sciences, Skromna 8, 20-704 Lublin, Poland.
Department of Analysis and Evaluation of Food Quality, Faculty of Food Science and Biotechnology, University of Life Sciences, Skromna 8, 20-704 Lublin, Poland.
出版信息
Food Chem. 2017 Apr 15;221:1361-1370. doi: 10.1016/j.foodchem.2016.11.018. Epub 2016 Nov 4.
The cultures of Saccharomyces cerevisiae were treated with pulsed electric fields (PEF) in order to obtain a maximum accumulation of selenium and zinc ions (simultaneously) in the biomass. The following concentrations: 100μgSe/ml and 150μgZn/ml medium were assumed to be optimal for the maximum accumulation of these ions, that is 43.07mg/gd.m. for selenium and 14.48mg/gd.m. for zinc, in the cultures treated with PEF. At optimal PEF parameters: electric field strength of 3kV/cm and pulse width of 10μs after the treatment of 20-h culture for 10min, the maximum accumulation of both ions in the yeast cells was observed. Application of PEF caused the increase of ions accumulation by 65% for selenium and 100% for zinc. Optimization of PEF parameters led to the further rise in the both ions accumulation resulting in over 2-fold and 2.5-fold higher concentration of selenium and zinc.
对酿酒酵母培养物进行脉冲电场（PEF）处理，以便在生物质中（同时）获得最大量的硒和锌离子积累。假定以下浓度：100μgSe/ml和150μgZn/ml培养基对于这些离子的最大积累是最佳的，即在经PEF处理的培养物中，硒为43.07mg/g干物质，锌为14.48mg/g干物质。在最佳PEF参数下：电场强度为3kV/cm，脉冲宽度为10μs，对20小时培养物处理10分钟后，观察到酵母细胞中两种离子的最大积累。施加PEF使硒离子积累增加65%，锌离子积累增加100%。PEF参数的优化导致两种离子积累进一步增加，使得硒和锌的浓度分别提高了2倍多和2.5倍。
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