Department of Analysis and Evaluation of Food Quality, Faculty of Food Science and Biotechnology, University of Life Sciences-Lublin, Skromna 8, Lublin, Poland.
J Microbiol Biotechnol. 2011 Jun;21(6):646-51.
Cultures of Saccharomyces cerevisiae were treated with pulsed electric fields to improve accumulation of zinc in the biomass. Under optimized conditions, that is, on 15 min exposure of the 20 h grown culture to PEFs of 1500 V and 10 microns pulse width, accumulation of zinc in the yeast biomass reached a maximum of 15.57 mg/g d.m. Under optimum zinc concentration (100 microgram/ml nutrient medium), its accumulation in the cells was higher by 63% in comparison with the control (without PEFs). That accumulation significantly correlated against zinc concentration in the medium. Neither multiple exposure of the cultures to PEFs nor intermittent supplementation of the cultures with zinc increased the zinc accumulation. The intermittent supplementation of the cultures with zinc and multiple exposures on PEFs could even reduce the accumulation efficiency, respectively, by 57% and 47%.
酵母 Saccharomyces cerevisiae 的培养物经脉冲电场处理以提高生物量中锌的积累。在优化条件下，即 20 小时生长培养物暴露于 1500 V 和 10 微米脉冲宽度的 PEF 15 分钟时，酵母生物质中锌的积累达到最大值 15.57 毫克/克干重。在最佳锌浓度（100 微克/毫升营养培养基）下，与对照（无 PEF）相比，细胞内锌的积累增加了 63%。这种积累与培养基中锌的浓度呈显著正相关。培养物多次暴露于 PEF 或间歇补充锌均不能增加锌的积累。间歇补充锌和多次暴露于 PEF 会分别使积累效率降低 57%和 47%。
