Góral Małgorzata, Pankiewicz Urszula
Department of Analysis and Food Quality Assessment, Faculty of Food Science and Biotechnology, University of Life Sciences, Skromna Street 8, 20-704, Lublin, Poland.
J Membr Biol. 2017 Oct;250(5):565-572. doi: 10.1007/s00232-017-9986-6. Epub 2017 Sep 4.
The aim of this study was to determine the effect of pulsed electric fields (PEF) on accumulation of magnesium ions in Lactobacillus rhamnosus B 442 cells. Under optimized conditions, this is, on 15 min exposure of the 20 h grown culture to PEF of the 2.0 kV/cm and 20 µs pulse width at concentration 400 μg Mg/mL medium, accumulation of magnesium in the biomass reached maximum 4.28 mg/g d.m. Optimization of PEF parameters caused an increase of magnesium concentration in the cells by 220% in comparison to the control not treated with PEF. Bacterial cell biomass enriched with Mg may be an alternative for pharmacological supplementation applied in deficiency of this cation.
本研究的目的是确定脉冲电场（PEF）对鼠李糖乳杆菌B 442细胞中镁离子积累的影响。在优化条件下，即20小时培养的菌液在浓度为400μg Mg/mL培养基中，于2.0 kV/cm和20μs脉冲宽度的PEF下暴露15分钟，生物量中镁的积累量达到最大值4.28 mg/g干重。与未用PEF处理的对照相比，PEF参数的优化使细胞中镁浓度增加了220%。富含镁的细菌细胞生物量可能是在这种阳离子缺乏时用于药物补充的一种替代物。
