Department of Analysis and Evaluation of Food Quality, Faculty of Food Sciences and Biotechnology, University of Life Sciences in Lublin, Skromna 8, 20-704 Lublin, Poland.
Faculty of Electrical Engineering, University of Ljubljana, Trzaska Cesta 25, 1000 Ljubljana, Slovenia.
Biomolecules. 2021 Jun 7;11(6):850. doi: 10.3390/biom11060850.
The aim of the study was to investigate the influence of a pulsed electric field (PEF) on the level of iron ion accumulation in cells and to select PEF conditions optimal for the highest uptake of this element. Iron ions were accumulated most efficiently when their source was iron (III) nitrate. When the following conditions of PEF treatment were used: voltage 1500 V, pulse width 10 μs, treatment time 20 min, and a number of pulses 1200, accumulation of iron ions in the cells from a 20 h-culture reached a maximum value of 48.01 mg/g dry mass. Application of the optimal PEF conditions thus increased iron accumulation in cells by 157% as compared to the sample enriched with iron without PEF. The second derivative of the FTIR spectra of iron-loaded and -unloaded yeast cells allowed us to determine the functional groups which may be involved in metal ion binding. The exposure of cells to PEF treatment only slightly influenced the biomass and cell viability. However, iron-enriched yeast (both with or without PEF) showed lower fermentative activity than a control sample. Thus obtained yeast biomass containing a high amount of incorporated iron may serve as an alternative to pharmacological supplementation in the state of iron deficiency.
本研究旨在探讨脉冲电场 (PEF) 对细胞中铁离子积累水平的影响，并选择最佳的 PEF 条件以实现对该元素的最高摄取。当铁离子的来源是硝酸铁时，其积累效率最高。当采用以下 PEF 处理条件时：电压 1500V、脉冲宽度 10μs、处理时间 20min 和脉冲数 1200，经过 20 小时培养的细胞中铁离子的积累达到最大值，为 48.01mg/g 干重。与未经 PEF 强化的含铁样品相比，最佳 PEF 条件的应用使细胞中铁的积累增加了 157%。负载铁和未负载铁的酵母细胞的傅里叶变换红外光谱（FTIR）二阶导数允许我们确定可能参与金属离子结合的功能基团。细胞暴露于 PEF 处理仅略微影响生物量和细胞活力。然而，与对照样品相比，富含铁的酵母（无论是否经过 PEF 处理）的发酵活性都较低。因此，获得的含有大量结合铁的酵母生物质可以作为缺铁状态下药物补充的替代物。
