Zhang Dongdong, Li Zhiling, Zhang Chunfang, Zhou Xue, Xiao Zhixing, Awata Takanori, Katayama Arata
Department of Civil Engineering, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan; EcoTopia Science Institute, Nagoya University, Chikusa, Nagoya 464-8603, Japan; Institute of Materials and Systems for Sustainability (IMaSS), Nagoya University, Chikusa, Nagoya 464-8603, Japan.
EcoTopia Science Institute, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
J Biosci Bioeng. 2017 Mar;123(3):364-369. doi: 10.1016/j.jbiosc.2016.10.010. Epub 2016 Dec 12.
A microbial fuel cell (MFC), with graphite electrodes as both the anode and cathode, was operated with a soil-free anaerobic consortium for phenol degradation. This phenol-degrading MFC showed high efficiency with a current density of 120 mA/m and a coulombic efficiency of 22.7%, despite the lack of a platinum catalyst cathode and inoculation of sediment/soil. Removal of planktonic bacteria by renewing the anaerobic medium did not decrease the performance, suggesting that the phenol-degrading MFC was not maintained by the planktonic bacteria but by the microorganisms in the anode biofilm. Cyclic voltammetry analysis of the anode biofilm showed distinct oxidation and reduction peaks. Analysis of the microbial community structure of the anode biofilm and the planktonic bacteria based on 16S rRNA gene sequences suggested that Geobacter sp. was the phenol degrader in the anode biofilm and was responsible for current generation.
一种以石墨电极作为阳极和阴极的微生物燃料电池（MFC），采用无土壤厌氧菌群运行以降解苯酚。尽管缺少铂催化剂阴极且未接种沉积物/土壤，但这种降解苯酚的MFC仍表现出高效性，电流密度为120 mA/m，库仑效率为22.7%。通过更新厌氧培养基去除浮游细菌并未降低性能，这表明降解苯酚的MFC不是由浮游细菌维持，而是由阳极生物膜中的微生物维持。对阳极生物膜的循环伏安分析显示出明显的氧化和还原峰。基于16S rRNA基因序列对阳极生物膜和浮游细菌的微生物群落结构分析表明，地杆菌属是阳极生物膜中的苯酚降解菌，并负责电流产生。
