Goto Yuko, Yoshida Naoko
Department of Biomedical Science, Chubu University.
Department of Civil and Environmental Engineering, Nagoya Institute of Technology.
J Gen Appl Microbiol. 2017 Jul 11;63(3):165-171. doi: 10.2323/jgam.2016.10.001. Epub 2017 May 2.
Anodes are crucial in determining the electricity recovery of microbial fuel cells (MFCs). In this study, graphene oxide (GO) was used as an anodic material for electricity recovery from artificial dialysis wastewater (ADWW). Anaerobic incubation of ADWW with GO for 21 days produced a hydrogel complex containing embedded microbial cells and microbially reduced GO (rGO). The rGO complex recovered 540 to 810 μA/cm of catalytic current from ADWW after 10 days of electrochemical cultivation at 200 mV (vs. Ag/AgCl), which was approximately thirty times higher than that recovered from graphite felt (GF), a representative anode in MFCs. High-throughput sequencing analysis of prokaryotic 16S rRNA genes revealed a predominance of the Geobacter genus (35% of all prokaryotic sequences identified), particularly in the rGO complex after 20 days of polarization. The superior electricity recovery of the rGO complex was attributable to enhanced direct electron transfer via a well-developed biofilm, while indirect electron transfer via an electron mediator occurred in culture using GF.
阳极对于确定微生物燃料电池（MFC）的电力回收至关重要。在本研究中，氧化石墨烯（GO）被用作从人工透析废水（ADWW）中回收电力的阳极材料。将ADWW与GO进行厌氧培养21天，产生了一种水凝胶复合物，其中包含嵌入的微生物细胞和微生物还原的GO（rGO）。在200 mV（相对于Ag/AgCl）下进行10天的电化学培养后，rGO复合物从ADWW中回收了540至810 μA/cm的催化电流，这大约是从MFCs中代表性阳极石墨毡（GF）回收电流的30倍。对原核生物16S rRNA基因的高通量测序分析表明，地杆菌属占主导地位（占所有鉴定的原核生物序列的35%），特别是在极化20天后的rGO复合物中。rGO复合物卓越的电力回收归因于通过发育良好的生物膜增强了直接电子转移，而在使用GF的培养中则通过电子介体发生间接电子转移。
