Nifant'ev Ilya, Besprozvannykh Victoria, Shlyakhtin Andrey, Tavtorkin Alexander, Legkov Sergei, Chinova Maria, Arutyunyan Irina, Soboleva Anna, Fatkhudinov Timur, Ivchenko Pavel
A.V. Topchiev Institute of Petrochemical Synthesis RAS, 29 Leninsky Pr., 119991 Moscow, Russia.
Chemistry Department, M.V. Lomonosov Moscow State University, 1-3 Leninskie Gory, 119991 Moscow, Russia.
Polymers (Basel). 2022 Oct 7;14(19):4203. doi: 10.3390/polym14194203.
Composite biocompatible scaffolds, obtained using the electrospinning (ES) technique, are highly promising for biomedical application thanks to their high surface area, porosity, adjustable fiber diameter, and permeability. However, the combination of synthetic biodegradable (such as poly(ε-caprolactone) PCL) and natural (such as gelatin Gt) polymers is complicated by the problem of low compatibility of the components. Previously, this problem was solved by PCL grafting and/or Gt cross-linking after ES molding. In the present study, composite fibrous scaffolds consisting of PCL and Gt were fabricated by the electrospinning (ES) method using non-functionalized or NHS-functionalized and hexafluoroisopropanol as a solvent. To provide covalent binding between and Gt macromolecules, NHS-functionalized methyl glutarate was synthesized and studied in model reactions with components of spinning solution. It was found that selective formation of amide bonds, which provide complete covalent bonding of Gt in PCL/Gt composite, requires the presence of weak acid. With the use of the optimized ES method, fibrous mats with different PCL/Gt ratios were prepared. The sample morphology (SEM), hydrolytic resistance (FT-IR), cell adhesion and viability (MTT assay), cell penetration (fluorescent microscopy), and mechanical characteristics of the samples were studied. -based films with a Gt content of 20 wt% have demonstrated the best set of properties.
通过静电纺丝（ES）技术获得的复合生物相容性支架，因其高表面积、孔隙率、可调节的纤维直径和渗透性，在生物医学应用方面具有很高的前景。然而，合成可生物降解聚合物（如聚己内酯PCL）和天然聚合物（如明胶Gt）的组合存在组分相容性低的问题，这使得二者的结合变得复杂。以前，这个问题通过ES成型后PCL接枝和/或Gt交联来解决。在本研究中，使用非功能化或NHS功能化的聚己内酯（PCL）和明胶（Gt），以六氟异丙醇作为溶剂，通过静电纺丝（ES）方法制备了复合纤维支架。为了使PCL和Gt大分子之间形成共价键，合成了NHS功能化的甲基戊二酸酯，并在与纺丝溶液组分的模型反应中对其进行了研究。结果发现，酰胺键的选择性形成需要弱酸的存在，而酰胺键能使Gt在PCL/Gt复合材料中实现完全共价键合。采用优化的ES方法，制备了不同PCL/Gt比例的纤维毡。研究了样品的形态（扫描电子显微镜）、耐水解性（傅里叶变换红外光谱）、细胞粘附和活力（MTT法）、细胞穿透（荧光显微镜）以及样品的机械特性。明胶含量为20 wt%的基于PCL的薄膜表现出了最佳的综合性能。
