3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
Group of Food Biochemistry, Marine Research Institute (IIM-CSIC), R/Eduardo Cabello, 6, CP 36208, Vigo, Pontevedra, Spain.
Carbohydr Polym. 2017 Feb 10;157:31-37. doi: 10.1016/j.carbpol.2016.09.050. Epub 2016 Sep 15.
Chondroitin sulfate (CS) was isolated from Scyliorhinus canicula (fin, head and skeleton), Prionace glauca (head), and Raja clavata (skeleton) by-products from fish processing industry using environmentally friendly processes. The molecular weight was determined by gel permeation chromatography and the sugar composition and sulfation position by NMR and SAX-HPLC after enzymatic digestion. The CSs showed a prevalent 6S GalNAc sulfation for the 3 species (4S/6S ratio lower than 1). A higher 6S sulfation was observed for P. glauca head and R. clavata skeleton (4S/6S ratio below 0.20) than for S. canicula (4S/6S ratio ca. 0.6). The existence of CS samples with such low 4S/6S ratio has only been observed before in a rare species of shark (Mitsukutina owatoni, globin shark). The good extraction yields achieved make S. canicula, P. glauca and R. clavata fish industry by-products a useful source of 6-sulfated chondroitin sulfate.
从鲨鱼（赤魟）、马鲛鱼（蓝圆鯵）和孔鳐（鳐鱼）的加工副产物中提取硫酸软骨素（CS），采用环保工艺。利用凝胶渗透色谱法测定了分子量，利用 NMR 和 SAX-HPLC 结合酶解分析了糖组成和硫酸化位置。这 3 种 CS 均以 6S-GalNAc 为主（4S/6S 比小于 1）。马鲛鱼头和孔鳐骨架中的 6S 硫酸化程度（4S/6S 比低于 0.20）高于赤魟（4S/6S 比约为 0.6）。此前仅在一种稀有的鲨鱼（小齿锯鲨）中观察到过如此低 4S/6S 比的 CS 样品。如此高的提取收率表明赤魟、马鲛鱼和孔鳐的鱼加工副产物是 6-硫酸软骨素的有用来源。
