Yu Hui, Yi Xiangzhou, Gao Xia, Ji Jun, Liu Zhongyuan, Xia Guanghua, Li Chuan, Zhang Xueying, Shen Xuanri
Hainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, Hainan University, Haikou 570228, China.
College of Food Science and Technology, Hainan University, Haikou 570228, China.
Foods. 2022 Mar 23;11(7):922. doi: 10.3390/foods11070922.
We isolated and characterized tilapia-head chondroitin sulfate (TH-CS) and explored its biological activity and mechanisms of action as an oral supplement for nonalcoholic fatty liver disease (NAFLD) induced by a high-fat diet (HFD) in mice. The results showed that treatment with TH-CS for 8 weeks alleviated the development of NAFLD, as evidenced by the notable improvement in liver damage, blood lipid accumulation and insulin resistance (IR). Meanwhile, TH-CS treatment reduced the expression of proinflammatory cytokines and normalized oxidative stress. Additionally, the analysis of 16S rDNA sequencing revealed that TH-CS could restore gut microbiota balance and increase the relative abundance of short-chain fatty acid (SCFA)-producing bacteria. Furthermore, SCFAs produced by related bacteria can further improve lipid metabolism and IR by regulating lipid synthesis signals. In conclusion, TH-CS is an effective dietary supplement for the prevention of NAFLD, and may serve as a potential supplementary treatment for lipid-related metabolic syndrome.
我们分离并鉴定了罗非鱼鱼头硫酸软骨素（TH-CS），并探讨了其作为口服补充剂对高脂饮食（HFD）诱导的小鼠非酒精性脂肪性肝病（NAFLD）的生物活性及作用机制。结果表明，TH-CS治疗8周可减轻NAFLD的发展，肝损伤、血脂积累和胰岛素抵抗（IR）的显著改善证明了这一点。同时，TH-CS治疗降低了促炎细胞因子的表达并使氧化应激恢复正常。此外，16S rDNA测序分析显示，TH-CS可恢复肠道微生物群平衡并增加产生短链脂肪酸（SCFA）细菌的相对丰度。此外，相关细菌产生的SCFAs可通过调节脂质合成信号进一步改善脂质代谢和IR。总之，TH-CS是预防NAFLD的有效膳食补充剂，可能作为脂质相关代谢综合征的潜在辅助治疗方法。
