Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Carbohydr Polym. 2017 Feb 10;157:325-334. doi: 10.1016/j.carbpol.2016.09.092. Epub 2016 Oct 1.
As a naturally-abundant biopolymer, chitosan (CS) exhibit pH-sensitive structural transformation within a narrow pH range. Integrating hydrophobic groups to CS molecules gives modified CS polymers with more adjustable pH responsiveness. In this paper, near-infrared (NIR) photoluminescent AgS QDs capped by long-chain carboxylic acid were synthesized and then conjugated with CS via esterification reaction. The anticancer drug doxorubicin (DOX) has an affinity for the hydrophobic oleoyl groups and was entrapped by them to produce AgS(DOX)@CS nanospheres. A variety of experiments were performed to characterize the nanospheres. In vitro and in vivo experiments showed that the nanospheres can release DOX at lowered pH in tumor cells and have high antitumor efficacy. In addition, the strong NIR signal derived from the encapsulated AgS QDs makes real-time monitoring of the nanosphere distribution in a body possible. This study provides a new CS-based nanocomposite drug carrier for efficient cancer therapy.
壳聚糖（CS）作为一种天然存在的生物聚合物，在很窄的 pH 范围内表现出 pH 敏感性的结构转变。将疏水性基团整合到 CS 分子中，赋予了具有更可调节 pH 响应性的改性 CS 聚合物。在本文中，合成了被长链羧酸封端的近红外（NIR）光致发光 AgS QDs，并通过酯化反应将其与 CS 偶联。抗癌药物阿霉素（DOX）与疏水性油酰基具有亲和力，并被其包封以产生 AgS（DOX）@CS 纳米球。进行了各种实验来表征纳米球。体外和体内实验表明，纳米球可以在肿瘤细胞的较低 pH 值下释放 DOX，并具有高效的抗肿瘤功效。此外，包裹的 AgS QDs 产生的强 NIR 信号使得能够实时监测纳米球在体内的分布。这项研究为高效癌症治疗提供了一种基于 CS 的新型纳米复合材料药物载体。
