Wales R G, Edirisinghe W R
School of Veterinary Studies, Murdoch University, Australia.
Reprod Fertil Dev. 1989;1(2):171-8. doi: 10.1071/rd9890171.
Estimates of the volume of fluid and the concentration of cations in the uterus were made by flushing the uterine cavity of mice mated to vasectomized males and measuring the concentrations of sodium and potassium cations in the recovered fluid. On day 1 following mating, large volumes of fluid (greater than 90 microL) were found in the uterus but, from days 2 to 5, only 2-5 microL of fluid were present. The ratio of sodium to potassium fell from 4.5:1 on day 1 to 1.8:1 on days 2 to 5 of pseudopregnancy, indicating that uterine fluid in the mouse has a high K+ content ranging from 35 mEq L-1 on day 1 to 75 mEq L-1 on day 5. Glucose, lactate and pyruvate in uterine flushings were also assayed and their concentration in uterine fluid calculated using the volumes found above. The level of all substrates was low on day 1 after mating. From day 2 onwards approximately 1 mM glucose was present in the fluids. The concentration of lactate was more variable, and peaked at 4 mM on day 2 of pseudopregnancy. In general, the concentration of pyruvate was 10% of the lactate value.
通过冲洗与输精管结扎雄性小鼠交配的雌性小鼠子宫腔，并测量回收液中钠和钾阳离子的浓度，来估算子宫内的液体量和阳离子浓度。交配后第1天，子宫内发现大量液体（超过90微升），但在第2至5天，仅存在2 - 5微升液体。钠钾比从第1天的4.5:1降至假孕第2至5天的1.8:1，这表明小鼠子宫液中钾离子含量很高，从第1天的35毫当量/升范围到第5天的75毫当量/升。还测定了子宫冲洗液中的葡萄糖、乳酸盐和丙酮酸盐，并根据上述发现的液体量计算它们在子宫液中的浓度。交配后第1天所有底物的水平都很低。从第2天起，液体中大约存在1毫摩尔葡萄糖。乳酸盐浓度变化更大，在假孕第2天达到4毫摩尔的峰值。一般来说，丙酮酸盐浓度是乳酸盐值的10%。
