Sakoff J A, Dunstan R H, Murdoch R N
Department of Biological Sciences, University of Newcastle, Callaghan, Australia.
Mol Reprod Dev. 1996 May;44(1):93-102. doi: 10.1002/(SICI)1098-2795(199605)44:1<93::AID-MRD11>3.0.CO;2-1.
Lipid extracts of whole uterine tissue from mice were examined by gas chromatography-mass spectrometry during days 2,3, and 4 pseudopregnancy (day 1 = copulatory plug) and following the artificial induction of the decidual cell reaction (DCR) on day 4. The range of lipids identified during pseudopregnancy and their percentage composition on day 2 included saturated fatty acids (SFA, 38%), monounsaturated fatty acids (MUFA, 20%), polyunsaturated fatty acids (PUFA, 17%), sterols (25%), long chain alcohols (0.12%), and alkylglycerols (0.11%). Of these, the main components were the fatty acids 16:0 (21%), 18:0 (14%), cis18:In-9 (14%), 18:2n-6 (8.5%), and cholesterol (24%). Although only subtle changes in the composition of uterine lipids occurred through days 2 and 3 of pseudopregnancy, more substantial changes were detected on day 4, at a time when the uterus normally initiates its transient "window of receptivity." Following induction of the DCR with the lectin Concanavalin A (Con A) at this time, even greater alterations in uterine lipid composition were observed. From 20 to 1,280 min post-Con A-treatment the percentage composition of SFA in the treated left uterine horn changed from 43% to 64%, sterols from 19% to 4%, PUFA from 15% to 10%, while MUFA remained unchanged at 23%. The lipid profile of the untreated right uterine horn of these animals was similar to that of the Con A-treated left uterine horn during the early stages. However, by 1,280 min substantial differences were observed, at a time corresponding with Con A-induced uterine growth. In contrast, differences in the lipid profile of Con A- and saline-treated uteri were observed at 320 min post-treatment, a time preceding Con A-induced uterine growth. Furthermore, the tissue concentration (nmol/mg dry weight) of SFA and sterols in uterine tissue decreased significantly following Con A treatment. The results suggest that uterine lipid changes are implicated in the development of uterine receptivity, and in the remodeling of uterine tissue for successful embryonic invasion and the establishment of pregnancy.
在假孕第2、3、4天（第1天=交配栓）以及在第4天人工诱导蜕膜细胞反应（DCR）之后，通过气相色谱-质谱联用仪对小鼠全子宫组织的脂质提取物进行了检测。在假孕期间鉴定出的脂质种类及其在第2天的百分比组成包括饱和脂肪酸（SFA，38%）、单不饱和脂肪酸（MUFA，20%）、多不饱和脂肪酸（PUFA，17%）、固醇（25%）、长链醇（0.12%）和烷基甘油（0.11%）。其中，主要成分是脂肪酸16:0（21%）、18:0（14%）、顺式18:1n-9（14%）、18:2n-6（8.5%）以及胆固醇（24%）。尽管在假孕的第2天和第3天子宫脂质组成仅发生了细微变化，但在第4天检测到了更显著的变化，此时子宫通常开始其短暂的“接受窗”。在此期间用凝集素伴刀豆球蛋白A（Con A）诱导DCR后，观察到子宫脂质组成有更大的改变。在Con A处理后20至1280分钟，处理过的左子宫角中SFA的百分比组成从43%变为64%，固醇从19%变为4%，PUFA从15%变为10%，而MUFA保持不变，为23%。这些动物未处理的右子宫角的脂质谱在早期与Con A处理过的左子宫角相似。然而，到1280分钟时观察到了显著差异，此时与Con A诱导的子宫生长相对应。相比之下，在处理后320分钟观察到Con A处理组和生理盐水处理组子宫的脂质谱存在差异，这是在Con A诱导子宫生长之前的时间。此外，Con A处理后子宫组织中SFA和固醇（nmol/mg干重）的组织浓度显著降低。结果表明，子宫脂质变化与子宫接受性的发展以及子宫组织为成功的胚胎着床和妊娠建立而进行的重塑有关。
