Bailey Melanie, Randall Elizabeth C, Costa Catia, Salter Tara L, Race Alan M, de Puit Marcel, Koeberg Mattijs, Baumert Mark, Bunch Josephine
Department of Chemistry, University of Surrey, Guildford, GU2 7XH, UK.
National Physical Laboratory, Teddington, TW11 0LW, UK; School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK.
Anal Methods. 2016 Apr 28;2016(16):3373-3382. doi: 10.1039/C6AY00782A. Epub 2016 Mar 24.
Liquid Extraction Surface Analysis (LESA) is a new, high throughput tool for ambient mass spectrometry. A solvent droplet is deposited from a pipette tip onto a surface and maintains contact with both the surface and the pipette tip for a few seconds before being re-aspirated. The technique is particularly suited to the analysis of trace materials on surfaces due to its high sensitivity and low volume of sample removal. In this work, we assess the suitability of LESA for obtaining detailed chemical profiles of fingerprints, oral fluid and urine, which may be used in future for rapid medical diagnostics or metabolomics studies. We further show how LESA can be used to detect illicit drugs and their metabolites in urine, oral fluid and fingerprints. This makes LESA a potentially useful tool in the growing field of fingerprint chemical analysis, which is relevant not only to forensics but also to medical diagnostics. Finally, we show how LESA can be used to detect the explosive material RDX in contaminated artificial fingermarks.
液相萃取表面分析（LESA）是一种用于常压质谱分析的新型高通量工具。一个溶剂小滴从移液器吸头沉积到一个表面上，并在重新吸回之前与该表面和移液器吸头保持接触几秒钟。由于其高灵敏度和少量的样品去除量，该技术特别适合于表面痕量物质的分析。在这项工作中，我们评估了LESA用于获取指纹、口腔液和尿液详细化学图谱的适用性，这些图谱未来可用于快速医学诊断或代谢组学研究。我们进一步展示了LESA如何用于检测尿液、口腔液和指纹中的非法药物及其代谢物。这使得LESA在不断发展的指纹化学分析领域成为一种潜在有用的工具，这不仅与法医学相关，也与医学诊断相关。最后，我们展示了LESA如何用于检测受污染人工指纹中的爆炸物RDX。
