Dumbleton Jenna, Agarwal Pranay, Huang Haishui, Hogrebe Nathaniel, Han Renzhi, Gooch Keith J, He Xiaoming
Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210 (USA); Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210 (USA).
Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210 (USA); Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210 (USA); Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210 (USA).
Cell Mol Bioeng. 2016 Jun;9(2):277-288. doi: 10.1007/s12195-016-0428-9. Epub 2016 Jan 26.
Advancements in tissue engineering require the development of new technologies to study cell behavior . This study focuses on stem cell behavior within various miniaturized three-dimensional (3D) culture conditions of alginate biomaterials modified with the Arg-Gly-Asp (RGD) peptide known for its role in cell adhesion/attachment. Human embryonic palatal mesenchyme (HEPM) cells, bone marrow derived mesenchymal stem cells (MSCs), and human adipose derived stem cells (ADSCs) were cultured on a flat hydrogel of different concentrations of alginate-RGD, and in the miniaturized 3D core of microcapsules with either a 2% alginate or 2% alginate-RGD shell. The core was made of 0%, 0.5%, or 2% alginate-RGD. Cell spreading was observed in all systems containing the RGD peptide, and the cell morphology was quantified by measuring the cell surface area and circularity. In all types of stem cells, there was a significant increase in the cell surface area ( < 0.05) and a significant decrease in cell circularity ( < 0.01) in alginate-RGD conditions, indicating that cells spread much more readily in environments containing the peptide. This control over the cell spreading within a 3D microenvironment can help to create the ideal biomimetic condition in which to conduct further studies on cell behavior.
组织工程学的进展需要开发新技术来研究细胞行为。本研究聚焦于在各种小型化三维（3D）培养条件下，经精氨酸 - 甘氨酸 - 天冬氨酸（RGD）肽修饰的藻酸盐生物材料中的干细胞行为，该肽以其在细胞黏附/附着中的作用而闻名。人胚胎腭间充质（HEPM）细胞、骨髓间充质干细胞（MSCs）和人脂肪来源干细胞（ADSCs）分别培养于不同浓度藻酸盐 - RGD的平面水凝胶上，以及外壳为2%藻酸盐或2%藻酸盐 - RGD的微型3D微胶囊核心中。核心由0%、0.5%或2%的藻酸盐 - RGD制成。在所有含RGD肽的体系中均观察到细胞铺展，且通过测量细胞表面积和圆形度对细胞形态进行量化。在藻酸盐 - RGD条件下，所有类型的干细胞的细胞表面积均显著增加（<0.05），细胞圆形度显著降低（<0.01），这表明细胞在含该肽的环境中更容易铺展。这种对3D微环境中细胞铺展的控制有助于创造理想的仿生条件，以便对细胞行为进行进一步研究。
