钙诱导超声处理的磷脂酰丝氨酸囊泡融合的温度依赖性
Temperature dependence of calcium-induced fusion of sonicated phosphatidylserine vesicles.
作者信息
Sun S T, Day E P, Ho J T
出版信息
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4325-8. doi: 10.1073/pnas.75.9.4325.
Abstract
We have measured the temperature dependence calcium-induced fusion of sonicated phosphatidylserine vesicles. The vesicles were incubated in the presence of calcium at a specified temperature until the resulting aggregation or fusion process had gone to completion. EDTA was then added and the resulting final size of the vesicle population was measured by using dynamic light scattering. This final size was plotted against incubation temperature to show the temperature dependence of calcium-induced fusion. This curve has a peak near 11 degrees C which may be associated with the phase transition of the sonicated phosphatidylserine vesicles in the presence of calcium prior to the aggregation or fusion process.
摘要
我们测量了钙诱导的超声处理的磷脂酰丝氨酸囊泡融合的温度依赖性。将囊泡在特定温度下于钙存在的情况下孵育，直至产生的聚集或融合过程完成。然后加入乙二胺四乙酸（EDTA），并使用动态光散射测量所得囊泡群体的最终大小。将此最终大小与孵育温度作图，以显示钙诱导融合的温度依赖性。该曲线在11摄氏度附近有一个峰值，这可能与在聚集或融合过程之前钙存在时超声处理的磷脂酰丝氨酸囊泡的相变有关。
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