Goll J, Carlson F D, Barenholz Y, Litman B J, Thompson T E
Biophys J. 1982 Apr;38(1):7-13. doi: 10.1016/S0006-3495(82)84524-4.
The dependence of phospholipid vesicle size on lipid composition is investigated by photon correlation spectroscopy. For each lipid composition prolonged ultracentrifugation was used to isolate a nearly uniform population of minimum-sized vesicles. The residual size variations in the samples were sufficient to cause polydispersity that made comparisons between samples difficult. Analyses of the data by the method of cumulants and by a method for approximating the particle size distributions directly are presented. The latter method made possible unambiguous comparisons that revealed small but systematic dependences of vesicle size on composition in vesicles containing mixtures of egg phosphatidylcholine and phosphatidylethanolamine, egg phosphatidylcholine and beef brain sphingomyelin, and in single lipid vesicles of egg phosphatidylcholine, dioleylphosphatidylcholine, and beef brain sphingomyelin. These size dependences are quantified within the resolution limits of the technique and their implications are discussed.
通过光子相关光谱法研究了磷脂囊泡大小对脂质组成的依赖性。对于每种脂质组成，使用长时间超速离心来分离出几乎均匀的最小尺寸囊泡群体。样品中残留的尺寸变化足以导致多分散性，使得样品之间的比较变得困难。本文介绍了通过累积量法和直接近似颗粒尺寸分布的方法对数据进行分析。后一种方法使得能够进行明确的比较，揭示了在含有鸡蛋磷脂酰胆碱和磷脂酰乙醇胺混合物的囊泡、鸡蛋磷脂酰胆碱和牛脑鞘磷脂混合物的囊泡以及鸡蛋磷脂酰胆碱、二油酰磷脂酰胆碱和牛脑鞘磷脂的单一脂质囊泡中，囊泡大小对组成的微小但系统的依赖性。在该技术的分辨率极限内对这些大小依赖性进行了量化，并讨论了其含义。
