Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Biosens Bioelectron. 2017 Jun 15;92:229-233. doi: 10.1016/j.bios.2017.02.005. Epub 2017 Feb 4.
In this paper, a facile and rapid fluorescence turn-on assay for fluorescent detection of ascorbic acid (AA) was developed by using the orange emission graphene quantum dots (GQDs). In the presence of horse radish peroxidase (HRP) and hydrogen peroxide (HO), catechol can be oxidized by hydroxyl radicals and converted to o-benzoquinone, which can significantly quench the fluorescence of GQDs. However, when AA present in the system, it can consume part of HO and hydroxyl radicals to inhibit the generation of o-benzoquinone, resulting in fluorescence recovery. Under the optimized experimental conditions, the fluorescence intensity was linearly correlated with the concentration of HO in the range of 3.33-500µM with a detection limit of 1.2µM. The linear detection for AA was in the range from 1.11 to 300µM with a detection limit of 0.32µM. The proposed method was applied to the determination of AA in human serum samples with satisfactory results.
本文采用橙色发射的石墨烯量子点（GQDs），开发了一种简便、快速的荧光开启法，用于荧光检测抗坏血酸（AA）。在辣根过氧化物酶（HRP）和过氧化氢（HO）存在下，儿茶酚可被羟基自由基氧化，并转化为邻苯醌，这会显著猝灭 GQDs 的荧光。然而，当体系中存在 AA 时，它可以消耗部分 HO 和羟基自由基，从而抑制邻苯醌的生成，导致荧光恢复。在优化的实验条件下，荧光强度与 HO 在 3.33-500µM 范围内呈线性相关，检测限为 1.2µM。AA 的线性检测范围为 1.11-300µM，检测限为 0.32µM。该方法应用于人血清样品中 AA 的测定，结果令人满意。
