Vannacci E, Granchi S, Belsito L, Roncaglia A, Biagi E
Department of Information Engineering (DINFO), University of Florence, Via Santa Marta 3, 50139 Florence, Italy.
Department of Information Engineering (DINFO), University of Florence, Via Santa Marta 3, 50139 Florence, Italy.
Ultrasonics. 2017 Mar;75:164-173. doi: 10.1016/j.ultras.2016.11.024. Epub 2016 Nov 30.
An ultrasonic probe consisting of two optical fiber-based miniaturized transducers for wideband ultrasound emission and detection is employed for the characterization of in vitro biological tissues. In the probe, ultrasound generation is obtained by thermoelastic emission from patterned carbon films in Micro-Opto-Mechanical-System (MOMS) devices mounted on the tip of an optical fiber, whereas acousto-optical detection is performed in a similar way by a miniaturized polymeric interferometer. The microprobe presents a wide, flat bandwidth that is a very attractive feature for ultrasonic investigation, especially for tissue characterization. Thanks to the very high ultrasonic frequencies obtained, the probe is able to reveal different details of the object under investigation by analyzing the ultrasonic signal within different frequencies ranges, as shown by specific experiments performed on a patterned cornstarch flour sample in vitro. This is confirmed by measurements executed to determine the lateral resolution of the microprobe at different frequencies of about 70μm at 120MHz. Moreover, measurements performed with the wideband probe in pulsed-echo mode on a histological finding of porcine kidney are presented, on which two different spectral signal processing algorithms are applied. After processing, the ultrasonic spectral features show a peculiar spatial distribution on the sample, which is expected to depend on different ultrasonic backscattering properties of the analyzed tissues.
一种由两个基于光纤的小型化换能器组成的超声探头,用于宽带超声发射和检测,被用于体外生物组织的表征。在该探头中,超声产生是通过安装在光纤尖端的微机电系统(MOMS)器件中图案化碳膜的热弹性发射实现的,而声光检测则由一个小型化聚合物干涉仪以类似方式进行。该微探头具有宽而平坦的带宽,这对于超声研究,特别是组织表征来说是一个非常有吸引力的特性。由于获得了非常高的超声频率,该探头能够通过分析不同频率范围内的超声信号来揭示被研究物体的不同细节,如在体外对图案化玉米淀粉面粉样品进行的特定实验所示。通过测量确定微探头在约120MHz不同频率下的横向分辨率约为70μm,这证实了上述结论。此外,还展示了使用宽带探头在脉冲回波模式下对猪肾组织切片进行测量的结果,并应用了两种不同的光谱信号处理算法。处理后,超声光谱特征在样品上呈现出独特的空间分布,预计这取决于所分析组织不同的超声后向散射特性。