Pelyhe Csilla, Kövesi Benjámin, Zándoki Erika, Kovács Balázs, Szabó-Fodor Judit, Mézes Miklós, Balogh Krisztián
Department of Nutrition, Szent István University , Páter Károly u. 1, H-2103 Gödöllő , Hungary.
Academy of Sciences-Kaposvár University , Mycotoxins in the Food Chain Research Group, Kaposvár , Hungary.
Acta Vet Hung. 2016 Dec;64(4):449-466. doi: 10.1556/004.2016.042.
The purpose of this study was to investigate the short-term effects of a single oral dose of T-2 and HT-2 toxin at 0.15, 0.33 and 1.82 mg kg body weight, or deoxynivalenol (DON) and 15-acetyl-DON at 0.13, 0.31 and 1.75 mg kg body weight in common carp. Conjugated dienes and trienes (the early markers of lipid peroxidation) were elevated in all DON-treated groups at the 16th hour, while thiobarbituric acid reactive substances (TBARS; termination marker) were increased at the highest dose of DON at the 16th and 24th hours. T-2 toxin did not cause changes in these parameters. Glutathione content and glutathione peroxidase activity showed higher levels at the 16th hour as the effect of both mycotoxins. The expression of glutathione peroxidase (GPx4) genes (gpx4a and gpx4b) revealed a dual response. Downregulation was observed at the 8th hour, followed by an induction at the 16th hour, at the lowest dose of both mycotoxins. Higher doses revealed long-drawn emergence and an elevation was observed only at the 24th hour. However, at the lowest and highest doses of DON or T-2 toxin the changes in gene expression were delayed, which may be related to the low oxidative stress response, as suggested by the expression profiles of the nrf2, keap1, gpx4a and gpx4b genes.
本研究的目的是调查以0.15、0.33和1.82毫克/千克体重的单剂量口服T-2毒素和HT-2毒素，或0.13、0.31和1.75毫克/千克体重的脱氧雪腐镰刀菌烯醇（DON）和15-乙酰基-DON对鲤鱼的短期影响。在第16小时，所有DON处理组中，共轭二烯和三烯（脂质过氧化的早期标志物）均升高，而在第16小时和第24小时，最高剂量的DON组中硫代巴比妥酸反应性物质（TBARS；终末标志物）增加。T-2毒素未引起这些参数的变化。作为两种霉菌毒素作用的结果，谷胱甘肽含量和谷胱甘肽过氧化物酶活性在第16小时显示出较高水平。谷胱甘肽过氧化物酶（GPx4）基因（gpx4a和gpx4b）的表达呈现双重反应。在两种霉菌毒素的最低剂量下，第8小时观察到下调，随后在第16小时诱导上调。较高剂量显示出延迟出现，仅在第24小时观察到升高。然而，在DON或T-2毒素的最低和最高剂量下，基因表达的变化延迟，这可能与nrf2、keap1、gpx4a和gpx4b基因的表达谱所表明的低氧化应激反应有关。
