Department of Nutrition, Szent István University, Gödöllő, Hungary.
Mycotoxins in the Food Chain Research Group, Hungarian Academy of Sciences, Kaposvár University, Szent István University, Kaposvár, Hungary.
J Anim Physiol Anim Nutr (Berl). 2020 Sep;104(5):1531-1539. doi: 10.1111/jpn.13343. Epub 2020 Mar 12.
The purpose of the present study was to evaluate the short-term effects of aflatoxin B (AFB ) and deoxynivalenol (DON) exposure on the expression of the genes encoding the glutathione redox system glutathione peroxidase 4a (gpx4a), glutathione peroxidase 4b (gpx4b), glutathione synthetase (gss) and glutathione reductase (gsr) and the oxidative stress response-related transcription factors Kelch-like ECH-associated protein 1 (keap1) and nuclear factor-erythroid 2 p45-related factor 2 (nrf2) in liver, kidney and spleen of common carp. During the 24-hr long experiment, three different doses (5 µg AFB and 110 µg DON; 7.5 µg AFB and 165 µg DON or 10 µg AFB and 220 µg DON/kg bw) were used. The results indicated that the co-exposure of AFB and DON initiated free radical formation in liver, kidney and spleen, which was suggested by the increase in Nrf2 dependent genes, namely gpx4a, gpx4b, gss and gsr. Expression of keap1 gene showed upregulation after 8 hr of mycotoxin exposure, and also upregulation of nrf2 gene was found in kidney after 8 hr of exposure, while in the liver, only slight differences were observed. The changes in the expression of the analysed genes suggest that level of reactive oxygen species reached a critical level where other signalling pathway was activated as described by the hierarchical model of oxidative stress.
本研究的目的是评估黄曲霉毒素 B (AFB) 和脱氧雪腐镰刀菌烯醇 (DON) 暴露对编码谷胱甘肽氧化还原系统基因表达的短期影响谷胱甘肽过氧化物酶 4a (gpx4a)、谷胱甘肽过氧化物酶 4b (gpx4b)、谷胱甘肽合成酶 (gss) 和谷胱甘肽还原酶 (gsr) 以及与氧化应激反应相关的转录因子 Kelch 样 ECH 相关蛋白 1 (keap1) 和核因子-红细胞 2 p45 相关因子 2 (nrf2) 在鲤鱼的肝脏、肾脏和脾脏中。在长达 24 小时的实验中，使用了三种不同的剂量 (5 µg AFB 和 110 µg DON；7.5 µg AFB 和 165 µg DON 或 10 µg AFB 和 220 µg DON/kg bw)。结果表明，AFB 和 DON 的共同暴露在肝脏、肾脏和脾脏中引发了自由基的形成，这表明 Nrf2 依赖性基因，即 gpx4a、gpx4b、gss 和 gsr 的增加。keap1 基因的表达在接触霉菌毒素 8 小时后上调，nrf2 基因在肾脏接触 8 小时后也上调，而在肝脏中，仅观察到轻微差异。分析基因表达的变化表明，活性氧的水平达到了一个临界点，其他信号通路被激活，如氧化应激的层次模型所描述的那样。
