Ishiguro Koji, Kurata Rie, Shimada Yoshikazu, Sameshima Yoto, Kume Takashi
Hokkaido Agricultural Research Center, NARO, Shinsei Minami 9-4, Memuro, Kasai-gun, Hokkaido 082-0081, Japan.
Kyushu Okinawa Agricultural Research Center, NARO, Yokoichi 6651-2, Miyakonojo, Miyazaki 885-0091, Japan.
Heliyon. 2016 Dec 7;2(12):e00201. doi: 10.1016/j.heliyon.2016.e00201. eCollection 2016 Dec.
Sweetpotato peptide (SPP) was prepared by enzyme digestion of sweetpotato protein from starch wastewater. Animal experiments assessed the effect of SPP on body weight, abdominal adipose tissue mass, serum lipids and adipocytokines. Body and liver weight and epididymal and mesenteric fat of mice fed a high-fat diet containing 0.5% or 5% SPP for 28 days were significantly lower than control mice. Triglyceride and cholesterol in VLDL and LDL and leptin levels were significantly lower in the serum of SPP-administered mice compared to control mice. Biomarker arrays showed that adiponectin, melanocyte-stimulating-hormone-alpha and neuromedin U were more than 1.5 times higher, while TNF-alpha was about 1.5 times lower in the livers of SPP-administered mice compared to control mice. These results suggest SPP mitigated leptin resistance in mice administered a high-fat diet, and maintained anorexigenic peptide levels. SPP administration may suppress lipogenesis by increasing adiponectin levels and decreasing TNF-alpha levels in adipocytes.
通过对淀粉废水中的甘薯蛋白进行酶解制备了甘薯肽（SPP）。动物实验评估了SPP对体重、腹部脂肪组织量、血脂和脂肪细胞因子的影响。喂食含0.5%或5% SPP的高脂饮食28天的小鼠的体重、肝脏重量以及附睾和肠系膜脂肪明显低于对照小鼠。与对照小鼠相比，给予SPP的小鼠血清中极低密度脂蛋白（VLDL）和低密度脂蛋白（LDL）中的甘油三酯和胆固醇以及瘦素水平显著降低。生物标志物阵列显示，与对照小鼠相比，给予SPP的小鼠肝脏中的脂联素、α-促黑素细胞激素和神经介素U高出1.5倍以上，而肿瘤坏死因子-α（TNF-α）则低约1.5倍。这些结果表明，SPP减轻了高脂饮食小鼠的瘦素抵抗，并维持了厌食肽水平。给予SPP可能通过提高脂联素水平和降低脂肪细胞中的TNF-α水平来抑制脂肪生成。
