Kim Ryeo-Ok, Choi Jin Soo, Kim Byoung-Chul, Kim Woo-Keun
System Toxicology Research Center, Korea Institute of Toxicology, Daejeon, 34111, South Korea.
Future Environmental Research Center, Korea Institute of Toxicology, Jinju, 52834, South Korea.
Bull Environ Contam Toxicol. 2017 Feb;98(2):183-189. doi: 10.1007/s00128-016-1995-0. Epub 2016 Dec 19.
Many studies of the toxic effects of zinc oxide nanoparticles (ZnO NPs) in aquatic organisms have been performed because of increasing ZnO NP use. However, the toxicological pathways are not understood. In this study, ZnO NPs were found to be more toxic than ZnSO to zebrafish larvae, but ZnO NP toxicity did not involve transcript alterations. Biological processes affected by ZnO NPs and ZnSO were investigated by performing ingenuity pathway analysis on differently expressed genes in larvae exposed to sub-lethal ZnO NP and ZnSO concentrations. We identified upregulated and downregulated differently expressed genes in fish exposed to ZnO NPs and ZnSO, and found that ZnO NPs slightly induced cell differentiation and pathways associated with the immune system and activated several key genes involved in cancer cell signaling. The results may be key to predicting and elucidating the mechanisms involved in ZnO NP and ZnSO toxicity in zebrafish larvae.
由于氧化锌纳米颗粒（ZnO NPs）的使用不断增加，已经对其在水生生物中的毒性作用进行了许多研究。然而，毒理学途径尚不清楚。在本研究中，发现ZnO NPs对斑马鱼幼体的毒性比ZnSO更大，但ZnO NP的毒性并不涉及转录改变。通过对暴露于亚致死浓度的ZnO NP和ZnSO的幼体中差异表达基因进行 Ingenuity 通路分析，研究了受ZnO NPs和ZnSO影响的生物学过程。我们鉴定了暴露于ZnO NPs和ZnSO的鱼类中上调和下调的差异表达基因，发现ZnO NPs轻微诱导细胞分化以及与免疫系统相关的通路，并激活了参与癌细胞信号传导的几个关键基因。这些结果可能是预测和阐明ZnO NP和ZnSO对斑马鱼幼体毒性作用机制的关键。
