Alkaladi Ali, Afifi Mohamed, Ali Haytham, Saddick Salina
University of Jeddah, College of Science, Department of Biology, Jeddah, Saudi Arabia.
University of Jeddah, College of Science, Department of Biochemistry, Jeddah, Saudi Arabia.
Saudi J Biol Sci. 2020 May;27(5):1296-1301. doi: 10.1016/j.sjbs.2020.01.010. Epub 2020 Jan 16.
This study was carried out to determine the biochemical and molecular potential effects of Zn-ONPs sub-lethal toxicity on the hormonal profile of (O. niloticus). One hundred and fifty juvenile female were used in this experiment; Ninety for determination of LC50 and other 60 fish were divided into 3 groups with 20 fish each (two replicate in each group). Group I used as control group whereas other groups treated with 1/20 and 1/30 of LC50 respectively for 4 days. Serum, pituitary gland, hepatic, pancreatic and muscular tissues were obtained for hormonal and molecular evaluation. Serum growth hormone (GH), thyroid stimulating hormone (TSH), triiodothyronine (T3), tetraiodothyronine (T4), follicular stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), testosterone and insulin hormones were significantly decreased with a significant increase in both Adrenocorticosteroid hormone (ACTH) and cortisol levels with no change in serum glucagon levels. On molecular levels there were a significant down regulation in transcriptional levels of GH, Insulin like growth factor I (IGF-I), insulin and Insulin receptor-A (IRA genes. These results suggested that, hormonal and molecular alterations can be used as an early biomarkers for Zn-ONPs toxicity in fish.
本研究旨在确定纳米氧化锌（Zn-ONPs）亚致死毒性对尼罗罗非鱼（O. niloticus）激素水平的生化和分子潜在影响。本实验使用了150尾幼年雌鱼；其中90尾用于测定半数致死浓度（LC50），另外60尾鱼分为3组，每组20尾（每组两个重复）。第一组作为对照组，其他两组分别用LC50的1/20和1/30处理4天。采集血清、垂体、肝脏、胰腺和肌肉组织进行激素和分子评估。血清生长激素（GH）、促甲状腺激素（TSH）、三碘甲状腺原氨酸（T3）、甲状腺素（T4）、促卵泡激素（FSH）、黄体生成素（LH）、雌二醇（E2）、睾酮和胰岛素激素水平显著降低，而促肾上腺皮质激素（ACTH）和皮质醇水平显著升高，血清胰高血糖素水平无变化。在分子水平上，GH、胰岛素样生长因子I（IGF-I）、胰岛素和胰岛素受体-A（IRA）基因的转录水平显著下调。这些结果表明，激素和分子变化可作为鱼类Zn-ONPs毒性的早期生物标志物。
