Department of Biology, James Madison University, Harrisonburg, Virginia 22807, USA.
Department of Public Health Sciences University of Virginia, Charlottesville, Virginia 22908, USA.
Sci Data. 2016 Dec 20;3:160117. doi: 10.1038/sdata.2016.117.
RNA sequencing transcriptome analysis using massively parallel next generation sequencing technology provides the capability to understand global changes in gene expression throughout a range of tissue samples. Development of the vertebrate retina requires complex temporal orchestration of transcriptional activation and repression. The chicken embryo (Gallus gallus) is a classic model system for studying developmental biology and retinogenesis. Existing retinal transcriptome projects have been critical to the vision research community for studying aspects of murine and human retinogenesis, however, there are currently no publicly available data sets describing the developing chicken retinal transcriptome. Here we used Illumina RNA sequencing (RNA-seq) analysis to characterize the mRNA transcriptome of the developing chicken retina in an effort to identify genes critical for retinal development in this important model organism. These data will be valuable to the vision research community for characterizing global changes in gene expression between ocular tissues and critical developmental time points during retinogenesis in the chicken retina.
使用大规模平行下一代测序技术进行 RNA 测序转录组分析,使我们能够了解一系列组织样本中基因表达的全局变化。脊椎动物视网膜的发育需要转录激活和抑制的复杂时间协调。鸡胚胎(Gallus gallus)是研究发育生物学和视网膜发生的经典模式系统。现有的视网膜转录组项目对于研究鼠和人类视网膜发生的各个方面的视觉研究社区至关重要,但是,目前尚无公开可用的数据组描述发育中的鸡视网膜转录组。在这里,我们使用 Illumina RNA 测序(RNA-seq)分析来描述发育中鸡视网膜的 mRNA 转录组,以鉴定该重要模式生物中视网膜发育所必需的基因。这些数据对于视觉研究社区来说非常有价值,可用于描述鸡视网膜发生过程中眼球组织之间以及关键发育时间点的基因表达的全局变化。