Schumacker Scott T, Chidester Chloe A M, Enke Raymond A, Marcello Matthew R
Department of Biology, James Madison University, USA.
Department of Biology, Pace University, USA.
Data Brief. 2019 May 24;25:104006. doi: 10.1016/j.dib.2019.104006. eCollection 2019 Aug.
Transcriptome analysis using next generation sequencing (NGS) technology provides the capability to understand global changes in gene expression throughout a range of tissue samples. The nematode is a well-established genetic system used for analyzing a number of biological processes. are a bacteria-eating soil nematode, and changes in bacterial diet have been shown to cause a number of physiological and molecular changes. Here we used Illumina RNA sequencing (RNA-seq) analysis to characterize the mRNA transcriptome of mixed populations fed differing strains of bacteria to further understand dietary changes at the molecular level. Raw FASTQ files for the RNA-seq libraries are deposited in the NCBI Sequence Read Archive (SRA) and have been assigned BioProject accession PRJNA412551.
使用下一代测序(NGS)技术进行转录组分析,能够了解一系列组织样本中基因表达的整体变化。线虫是一个成熟的遗传系统,用于分析许多生物学过程。秀丽隐杆线虫是一种以细菌为食的土壤线虫,已表明细菌食物的变化会导致许多生理和分子变化。在这里,我们使用Illumina RNA测序(RNA-seq)分析来表征喂食不同菌株细菌的混合秀丽隐杆线虫群体的mRNA转录组,以进一步了解分子水平上的饮食变化。RNA-seq文库的原始FASTQ文件存放在NCBI序列读取存档(SRA)中,并已被分配生物项目编号PRJNA412551。