Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Napoli, Italy.
Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di ricerca per la genomica vegetale, 29017 Fiorenzuola d'Arda, Italy.
Sci Data. 2016 Dec 20;3:160115. doi: 10.1038/sdata.2016.115.
Posidonia oceanica is an endemic seagrass in the Mediterranean Sea, where it provides important ecosystem services and sustains a rich and diverse ecosystem. P. oceanica meadows extend from the surface to 40 meters depth. With the aim of boosting research in this iconic species, we generated a comprehensive RNA-Seq data set for P. oceanica by sequencing specimens collected at two depths and two times during the day. With this approach we attempted to capture the transcriptional diversity associated with change in light and other depth-related environmental factors. Using this extensive data set we generated gene predictions and identified an extensive catalogue of potential Simple Sequence Repeats (SSR) markers. The data generated here will open new avenues for the analysis of population genetic features and functional variation in P. oceanica. In total, 79,235 contigs were obtained by the assembly of 70,453,120 paired end reads. 43,711 contigs were successfully annotated. A total of 17,436 SSR were identified within 13,912 contigs.
地中海特有植物海草波西多尼亚海草为该海域提供了重要的生态系统服务，并维持着丰富多样的生态系统。波西多尼亚海草草原从水面延伸到 40 米深的地方。为了促进对这一标志性物种的研究，我们通过对白天两个不同深度采集的两个样本进行测序，生成了一个全面的波西多尼亚海草 RNA-Seq 数据集。通过这种方法，我们试图捕捉与光照变化和其他深度相关环境因素相关的转录多样性。利用这个广泛的数据集，我们生成了基因预测，并确定了广泛的潜在简单重复序列（SSR）标记目录。在这里生成的数据将为波西多尼亚海草的种群遗传特征和功能变异分析开辟新的途径。通过组装 70453120 对末端读取，共获得 79235 个连续序列。成功注释了 43711 个连续序列。在 13912 个连续序列中鉴定出 17436 个 SSR。
