Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210023, China.
Sci Data. 2018 Jun 5;5:180093. doi: 10.1038/sdata.2018.93.
Henosepilachna vigintioctopunctata is a vegetable pest that has spread worldwide. It belongs to the Coccinellidae family, whose members exhibit remarkable diversity, both in terms of their diets and the colored spots that appear on the elytra in the adult stage. Transcriptomic data from H. vigintioctopunctata at different life stages would be useful for further investigating the genetic basis of this dietary diversity and the formation of the colored spots in ladybird beetles, as well as revealing the population dynamics of H. vigintioctopunctata, which could be useful in pest control. Here, we generated a comprehensive RNA-seq data set (a total of ~24 Gb of clean data) for H. vigintioctopunctata by sequencing samples collected at different life stages. We characterized the transcriptomes of each of the four life stages (egg, larva, pupa, adult) and generated a high-coverage pool by combining all the RNA-seq reads. Furthermore, we identified a catalog of simple sequence repeat (SSR) markers. This represents the first study to collect transcriptome data from all life stages of a ladybird beetle.
二十八星瓢虫是一种世界性分布的蔬菜害虫，属于瓢虫科，其成员在饮食和成虫鞘翅上出现的彩色斑点方面表现出显著的多样性。不同发育阶段二十八星瓢虫的转录组数据可用于进一步研究这种饮食多样性和瓢虫彩色斑点形成的遗传基础，以及揭示二十八星瓢虫的种群动态，这对害虫防治可能是有用的。在这里，我们通过对不同发育阶段采集的样本进行测序，生成了一个全面的 RNA-seq 数据集（总共约 24GB 的清洁数据）。我们对四个发育阶段（卵、幼虫、蛹、成虫）的转录组进行了特征描述，并通过组合所有的 RNA-seq 读数生成了一个高覆盖度的文库。此外，我们还鉴定了一个简单重复序列（SSR）标记目录。这是首次从瓢虫的所有发育阶段收集转录组数据的研究。
