Golshan Masoumeh, Rezaei Kalantary Roshanak, Nasseri Simin, Farzadkia Mahdi, Esrafili Ali, Gharibzadeh Farzaneh, Karimi Takanlu Leila, Alvani Vida
Department of Environmental Health Engineering, School of Public Health, Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Environmental Health Engineering, Faculty of Public Health, Iran University of Medical Sciences, Tehran, Iran E-mail:
Water Sci Technol. 2016 Dec;74(12):2879-2888. doi: 10.2166/wst.2016.450.
In the current study, there are some considerable issues which focused on: (1) the production of biosurfactant, (2) its correlation with the initial bacterial inoculum in the liquid phase and (3) its effect on polycyclic hydrocarbon (PAH) bioremediation performance. Therefore, two strains Pseudomonas facilis and Pseudomonas spp. were able to form a large clear zone diameter on the oil surface. Phenanthrene (PHE) was also utilized as a sole substrate. Furthermore, biosurfactant production (BP) was detected by methylene blue analysis procedure and surface tension (ST) lowering. The capacity of these two strains to lower the aqueous ST was 29 and 30.7 from 72 mN m by 6 days for P. facilis and Pseudomonas spp., respectively. Higher initial bacterial inoculation led to an improvement in PHE removal (P < 0.0001). But there was no significant correlation between either PHE solubility or BP and the initial bacterial inoculation. The removal efficiency of 88.44% within 21 days confirms that the inoculation of P. facilis led to the considerable BP for removal of PAHs from contaminated water.
在当前研究中，存在一些值得关注的重要问题：（1）生物表面活性剂的产生；（2）其与液相中初始细菌接种量的相关性；（3）其对多环芳烃（PAH）生物修复性能的影响。因此，两株菌株，即易变假单胞菌和假单胞菌属，能够在油表面形成较大的透明圈直径。菲（PHE）也被用作唯一底物。此外，通过亚甲基蓝分析程序和表面张力（ST）降低来检测生物表面活性剂的产生（BP）。这两株菌株降低水相ST的能力，易变假单胞菌和假单胞菌属分别在6天时从72 mN/m降至29和30.7。较高的初始细菌接种量导致菲去除率提高（P < 0.0001）。但菲的溶解度或BP与初始细菌接种量之间均无显著相关性。21天内88.44%的去除效率证实，接种易变假单胞菌导致产生大量用于从污染水中去除多环芳烃的生物表面活性剂。
