D'Incau Emmeline, Ouvrard Stéphanie, Devers-Lamrani Marion, Jeandel Carole, Mohamed Chems Eddine, Henry Sonia
Université de Lorraine, INRAE, LSE, 54000, Nancy, France.
Agroécologie, AgroSup Dijon, CNRS, INRAE, Université de Bourgogne Franche-Comté, Dijon, France.
Biodegradation. 2024 Aug;35(5):719-737. doi: 10.1007/s10532-024-10073-6. Epub 2024 Mar 22.
Bioremediation is considered to be an effective treatment for hydrocarbon removal from polluted soils. However, the effectiveness of this treatment is often limited by the low availability of targeted contaminants. Biosurfactants produced by some microorganisms can increase organic compound solubility and might then overcome this limitation. Two different inocula producers of biosurfactants (Burkholderia thailandensis E264 and SHEMS1 microbial consortium isolated from a hydrocarbon-contaminated soil) were incubated in Bushnell-Haas medium supplemented with hydrocarbons to investigate their biodegradation potential. Experimental results showed their ability to degrade 9.1 and 6.1% of hydrocarbons respectively after 65 days of incubation with an initial total hydrocarbon concentration of 16 g L. The biodegradation was more effective for the light and medium fractions (C10 to C36). B. thailandensis and SHEMS1 consortium produced surfactants after 14 days of culture during the stationary phase with hydrocarbons as the sole carbon and energy source. However, biosurfactant production did not appear to directly increase hydrocarbon degradation efficiency. The complexity and recalcitrance of hydrocarbon mixture used in this study appeared to continue to limit its biodegradation even in the presence of biosurfactants. In conclusion, B. thailandensis and SHEMS1 consortium can degrade recalcitrant hydrocarbon compounds and are therefore good candidates for the bioremediation of environments polluted by total hydrocarbons.
生物修复被认为是从污染土壤中去除碳氢化合物的有效处理方法。然而，这种处理方法的有效性常常受到目标污染物可利用性低的限制。一些微生物产生的生物表面活性剂可以增加有机化合物的溶解度，从而可能克服这一限制。将两种不同的生物表面活性剂产生菌接种物（泰国伯克霍尔德菌E264和从碳氢化合物污染土壤中分离出的SHEMS1微生物群落）在添加了碳氢化合物的布什内尔-哈斯培养基中培养，以研究它们的生物降解潜力。实验结果表明，在初始总碳氢化合物浓度为16 g/L的情况下培养65天后，它们分别能够降解9.1%和6.1%的碳氢化合物。生物降解对轻质和中质馏分（C10至C36）更为有效。泰国伯克霍尔德菌和SHEMS1菌落在以碳氢化合物作为唯一碳源和能源的静止期培养14天后产生了表面活性剂。然而，生物表面活性剂的产生似乎并没有直接提高碳氢化合物的降解效率。本研究中使用的碳氢化合物混合物的复杂性和难降解性似乎即使在存在生物表面活性剂的情况下仍继续限制其生物降解。总之，泰国伯克霍尔德菌和SHEMS1菌落在可降解难降解的碳氢化合物化合物，因此是被总碳氢化合物污染的环境进行生物修复的良好候选者。
