Steiniger Birte S, Stachniss Vitus, Wilhelmi Verena, Seiler Anja, Lampp Katrin, Neff Andreas, Guthe Michael, Lobachev Oleg
Department of Immunobiology, Institute of Anatomy and Cell Biology, Universität Marburg, Marburg, Germany.
Dental Clinics, Histology Laboratory, Universität Marburg, Marburg, Germany.
PLoS One. 2016 Dec 20;11(12):e0168173. doi: 10.1371/journal.pone.0168173. eCollection 2016.
The arrangement of microvessels in human bone marrow is so far unknown. We combined monoclonal antibodies against CD34 and against CD141 to visualise all microvessel endothelia in 21 serial sections of about 1 cm2 size derived from a human iliac crest. The specimen was not decalcified and embedded in Technovit® 9100. In different regions of interest, the microvasculature was reconstructed in three dimensions using automatic methods. The three-dimensional models were subject to a rigid semiautomatic and manual quality control. In iliac crest bone marrow, the adipose tissue harbours irregularly distributed haematopoietic areas. These are fed by networks of large sinuses, which are loosely connected to networks of small capillaries prevailing in areas of pure adipose tissue. Our findings are compatible with the hypothesis that capillaries and sinuses in human iliac crest bone marrow are partially arranged in parallel.
迄今为止，人类骨髓中微血管的排列情况尚不清楚。我们将抗CD34和抗CD141的单克隆抗体结合起来，以观察来自人类髂嵴的21个面积约为1平方厘米的连续切片中的所有微血管内皮细胞。标本未脱钙，包埋于Technovit® 9100中。在不同的感兴趣区域，使用自动方法对微血管系统进行三维重建。三维模型经过严格的半自动和手动质量控制。在髂嵴骨髓中，脂肪组织含有分布不规则的造血区域。这些区域由大血窦网络供血，大血窦与纯脂肪组织区域中占主导的小毛细血管网络松散相连。我们的研究结果与以下假设相符：人类髂嵴骨髓中的毛细血管和血窦部分呈平行排列。
