Chu Shuang, Wang Li, Mao Xiao-Dong, Peng Wen
Laboratory of Renal Disease, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Cell Physiol Biochem. 2016;40(6):1354-1366. doi: 10.1159/000453188. Epub 2016 Dec 19.
BACKGROUND/AIMS: Endothelial dysfunction is a major factor in the progression of chronic kidney disease, which correlates with oxidative stress and NO deficiency. Huangqi decoction (HQD) is a potential anti-oxidant ingredient in renoprotection. However, the underlying mechanisms remained identified. Therefore, we investigated whether HQD exhibit improvement in endothelial dysfunction in the 5/6 nephrectomy (Nx) rat model.
Male Wistar rats (180 - 250 g) were divided into sham, Nx and Nx + HQD (0.05, 0.15 and 0.45 g/kg) group, respectively. Renal function and histology were examined with ELISA and Immunohistochemical analysis. Endothelium-dependent relaxation of rat aortas was investigated by isometric tension recordings. Oxidative stress and NO bioavailability were detected by ELISA, DHE-staining, DAF-2 staining and western blotting.
Compared with Nx rats, HQD treatment reversed the functional and structural changes of kidney significantly. Besides, endothelium-dependent relaxation of rat aortas was also improved by HQD treatment. NADPH oxidase and ROS generation were inhibited while NO bioavailability was enhanced.
HQD can act as a potent prescription for the treatment of endothelium related vascular complications.
背景/目的：内皮功能障碍是慢性肾脏病进展的主要因素，与氧化应激和一氧化氮（NO）缺乏相关。黄芪汤（HQD）是一种具有潜在抗氧化作用的肾脏保护成分。然而，其潜在机制尚待明确。因此，我们研究了黄芪汤是否能改善5/6肾切除（Nx）大鼠模型的内皮功能障碍。
雄性Wistar大鼠（180 - 250 g）分别分为假手术组、Nx组和Nx + HQD组（0.05、0.15和0.45 g/kg）。通过酶联免疫吸附测定（ELISA）和免疫组织化学分析检测肾功能和组织学。通过等长张力记录研究大鼠主动脉的内皮依赖性舒张。通过ELISA、二氢乙锭（DHE）染色、二氨基荧光素-2（DAF-2）染色和蛋白质印迹法检测氧化应激和NO生物利用度。
与Nx大鼠相比，HQD治疗显著逆转了肾脏的功能和结构变化。此外，HQD治疗还改善了大鼠主动脉的内皮依赖性舒张。烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶和活性氧（ROS）生成受到抑制，而NO生物利用度增强。
黄芪汤可作为治疗内皮相关血管并发症的有效方剂。
