Blaskovic Marko, Gabrić Dragana, Coleman Nichola J, Slipper Ian J, Mladenov Mitko, Gjorgievska Elizabeta
1Department of Oral Surgery,School of Dental Medicine, Faculty of Dentistry,University of Zagreb,Republic of Croatia, Ivana Gundulića 5, 10000, Zagreb,Croatia.
2Faculty of Engineering and Science,University of Greenwich,Maritime, Gillingham, Chatham ME4 4TB, Central Ave, Gillingham, Chatham ME4,UK.
Microsc Microanal. 2016 Dec;22(6):1170-1178. doi: 10.1017/S1431927616011971.
The aim of the present study was to compare dynamics of the bone healing process after different types of osteotomies. In total, 24 Wistar rats were subjected to different types of osteotomy performed with standard steel bur, piezosurgery, contact, and non-contact Erbium:yttrium-aluminum-garnet (Er:YAG) laser ablation. The animals were randomly divided into four groups, to be euthanized immediately after the procedure, or at 1, 2, or 3 weeks after surgery. The obtained bone samples were analyzed by scanning electron microscopy (SEM). Immediately after surgery, there were significant differences in the appearance of the bone defects, with presence of bone fragments and debris after standard steel bur preparation, compared with the clean smooth walls and relatively sharp edges in all other groups. The initial bone formation in defects prepared by piezosurgery was observed to be the most rapid. After 3 weeks, all bone defects were completely restored; although, differences in the healing pattern were noted, with a modest initial delay in healing after laser preparation. The first stage of the bone healing process was delayed when contact and non-contact Er:YAG laser modes were used and accelerated by piezosurgery; however, the results after 3 weeks demonstrated similar restitution of defects in all tested groups.
本研究的目的是比较不同类型截骨术后骨愈合过程的动态变化。总共24只Wistar大鼠接受了不同类型的截骨术，分别使用标准钢钻、压电手术、接触式和非接触式铒：钇铝石榴石（Er:YAG）激光消融进行。动物被随机分为四组，在手术后立即或术后1、2或3周实施安乐死。所获得的骨样本通过扫描电子显微镜（SEM）进行分析。手术后立即观察到，骨缺损的外观存在显著差异，与其他所有组中干净光滑的壁面和相对锐利的边缘相比，标准钢钻制备后存在骨碎片和碎屑。观察到压电手术制备的缺损处初始骨形成最为迅速。3周后，所有骨缺损均完全恢复；尽管注意到愈合模式存在差异，激光制备后愈合初期有适度延迟。使用接触式和非接触式Er:YAG激光模式时，骨愈合过程的第一阶段延迟，而压电手术则加速了这一过程；然而，3周后的结果表明，所有测试组的缺损恢复情况相似。
