Pardo-Planas Oscar, Prade Rolf A, Wilkins Mark R
Department of Biosystems and Agricultural Engineering, Oklahoma State University, 111 Agriculture Hall, Stillwater, OK, 74078, USA.
Department of Microbiology and Molecular Genetics, Oklahoma State University, 307 Life Sciences East, Stillwater, OK, 74078, USA.
J Ind Microbiol Biotechnol. 2017 Feb;44(2):247-257. doi: 10.1007/s10295-016-1884-x. Epub 2016 Dec 20.
Aryl alcohol oxidase (MtGloA) is an enzyme that belongs to the ligninolytic consortium and can play an important role in the bioenergy industry. This study investigated production of an MtGloA client enzyme by a mutant strain of Aspergillus nidulans unable to synthesize its own pyridoxine. Pyridoxine limitation can be used to control cell growth, diverting substrate to protein production. In agitated culture, enzyme production was similar when using media with 1 mg/L and without pyridoxine (26.64 ± 6.14 U/mg mycelia and 26.14 ± 8.39 U/mg mycelia using media with and without pyridoxine, respectively). However, the treatment lacking pyridoxine had to be supplemented with pyridoxine after 156 h of fermentation to sustain continued enzyme production. Use of extremely diluted pyridoxine levels allowed reduced fungal growth while maintaining steady enzyme production. Concentrations of 9 and 13.5 µg/L pyridoxine allowed MtGloA production with a growth rate of only 5% of that observed when using the standard 1 mg/L pyridoxine media.
芳基醇氧化酶（MtGloA）是一种属于木质素分解菌群的酶，在生物能源产业中可发挥重要作用。本研究调查了一株无法合成自身吡哆醇的构巢曲霉突变株对MtGloA客户酶的生产情况。吡哆醇限制可用于控制细胞生长，将底物转向蛋白质生产。在搅拌培养中，使用含1 mg/L吡哆醇的培养基和不含吡哆醇的培养基时，酶产量相似（分别使用含和不含吡哆醇的培养基时，酶产量为26.64±6.14 U/mg菌丝体和26.14±8.39 U/mg菌丝体）。然而，缺乏吡哆醇的处理在发酵156小时后必须补充吡哆醇以维持持续的酶生产。使用极低稀释度的吡哆醇水平可减少真菌生长，同时保持稳定的酶生产。9和13.5 µg/L的吡哆醇浓度可实现MtGloA的生产，其生长速率仅为使用标准1 mg/L吡哆醇培养基时观察到的生长速率的5%。
