Department of Biosystems and Agricultural Engineering, 111 Agriculture Hall, Oklahoma State University, Stillwater, OK 74078, USA.
Department of Microbiology and Molecular Genetics, 307 Life Sciences East, Oklahoma State University, Stillwater, OK 74078, USA.
Bioresour Technol. 2018 May;255:149-155. doi: 10.1016/j.biortech.2018.01.098. Epub 2018 Feb 4.
An A. nidulans strain with a pyridoxine marker was used for continuous production of aryl alcohol oxidase (AAO) in a trickle bed reactor (TBR). Modified medium with reduced zinc, no copper, and 5 g/L ascorbic acid that reduced melanin production and increased AAO productivity under growth limited conditions was used. Two air flow rates, 0.11 L/min (0.1 vvm) and 1.1 L/min (1.0 vvm) were tested. More melanin formation and reduced protein productivity were observed with air flow rate of 1.1 L/min. Three random packings were used as support for the fungus inside the TBR column, two of which were hydrophobic and one which was hydrophilic, and three different dilution rates were tested. The use of GEA BCN 030 hydrophobic packing resulted in greater AAO yield and productivity than the other packings. Increasing dilution rates favored melanin formation and citric, lactic and succinic acid accumulation, which decreased AAO yield and productivity.
一株带有吡哆醇标记的构巢曲霉菌株被用于在滴流床反应器（TBR）中连续生产芳醇氧化酶（AAO）。使用了改良的培养基，其中锌的含量降低，没有铜，并且添加了 5g/L 的抗坏血酸，这可以减少黑色素的产生并在生长受限的条件下提高 AAO 的生产力。测试了两种空气流速，0.11L/min（0.1vvm）和 1.1L/min（1.0vvm）。空气流速为 1.1L/min 时，观察到更多的黑色素形成和降低的蛋白质生产力。三种随机填充物被用作 TBR 柱内真菌的支撑物，其中两种是疏水性的，一种是亲水性的，并测试了三种不同的稀释率。使用 GEA BCN 030 疏水性包装的结果导致 AAO 产率和生产力大于其他包装。增加稀释率有利于黑色素形成以及柠檬酸、乳酸和琥珀酸的积累，这会降低 AAO 的产率和生产力。
