Keramati Sara, Pirdashti Hemmatollah, Babaeizad Valliollah, Dehestani Ali
Department of Agronomy, Genetic and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University , Sari , Iran.
Department of Phytopathology, Sari Agricultural Sciences and Natural Resources University, Sari, Iran , Km 9 Farah Abad Road, P.O. Box: 578, Sari , Iran.
Acta Biol Hung. 2016 Dec;67(4):412-423. doi: 10.1556/018.67.2016.4.7.
Essential oil content and oil composition of paclobutrazol treated sweet basil (Ocimum basilicum L.) plant inoculated with Piriformospora indica under salt stress were investigated by GC-MS. The results show a slight increase in essential oil content when basil plants subjected to moderate salinity stress (3 dS m of NaCl). It decreased signifiicantly with increasing salinity level to 9 dS m. The findings revealed that leaf area, above ground and leaf dry weights, essential oil content and yield were significantly affected by P. indica inoculation, however paclobutrazol application significantly influenced essential oil yield but not content. Fungal symbiosis as well as paclobutrazol application ameliorated the negative effects of salinity on dry matter and essential oil yield. The main constituents found in the volatile oil of O. basilicum in control treatment were Geranial (26.03%), Neral (24.88%) and Estragole (24.78%). The compounds concentrations showed some differences in P. indica and paclobutrazol treatments. The results demonstrate that micorrhiza-like fungi concomitantly increase essential oil production and biomass in sweet basil, a medicinal herb rich in commercially valuable essential oils.
通过气相色谱 - 质谱联用仪（GC - MS）研究了在盐胁迫下，用多效唑处理并接种印度梨形孢的甜罗勒（Ocimum basilicum L.）植株的精油含量和油成分。结果表明，当罗勒植株遭受中度盐胁迫（3 dS m的NaCl）时，精油含量略有增加。随着盐度水平增加到9 dS m，精油含量显著下降。研究结果显示，接种印度梨形孢对叶面积、地上部分和叶片干重、精油含量及产量有显著影响，然而，施用多效唑对精油产量有显著影响，但对含量无显著影响。真菌共生以及施用多效唑减轻了盐度对干物质和精油产量的负面影响。对照处理中甜罗勒挥发油中的主要成分是香叶醛（26.03%）、橙花醛（24.88%）和草蒿脑（24.78%）。在印度梨形孢和多效唑处理中，这些化合物的浓度存在一些差异。结果表明，类菌根真菌能同时提高富含商业价值精油的药用植物甜罗勒的精油产量和生物量。
