Department of Materials Science and Engineering, Yonsei University , Seoul 03722, Korea.
Biomacromolecules. 2017 Jan 9;18(1):27-35. doi: 10.1021/acs.biomac.6b01153. Epub 2016 Dec 21.
Although self-assembled peptide nanostructures (SPNs) have shown potential as promising biomaterials, there is a potential problem associated with the extremely slow hydrolysis rate of amide bonds. Here, we report the development of cell-penetrating cross-β SPNs with a controllable biodegradation rate. The designed self-assembling β-sheet peptide incorporating a hydrolyzable ester bond (self-assembling depsipeptide; SADP) can be assembled into bilayer β-sandwich one-dimensional (1D) fibers similarly to conventional β-sheet peptides. The rate of hydrolysis can be controlled by the pH, temperature, and structural characteristics of the ester unit. The 1D fiber of the SADP transforms into vesicle-like 3D structures when the hydrophilic cell-penetrating peptide segment is attached to the SADP segment. Efficient cell internalization of the 3D nanostructures was observed, and we verified the intracellular degradation and disassembly of the biodegradable nanostructures. This study illustrates the potential of biodegradable cross-β SPNs and provides a valuable toolkit that can be used with self-assembling peptides.
虽然自组装肽纳米结构 (SPN) 已显示出作为有前途的生物材料的潜力，但与酰胺键的极慢水解速率相关存在一个潜在问题。在这里，我们报告了具有可控生物降解速率的穿透细胞的交叉-β SPN 的开发。所设计的自组装包含可水解酯键的β-折叠肽（自组装去肽；SADP）可以像传统的β-折叠肽一样组装成双层β-夹层一维（1D）纤维。酯单元的 pH 值、温度和结构特征可以控制水解速率。当亲水性穿透细胞肽段连接到 SADP 段时，SADP 的 1D 纤维转变为类似囊泡的 3D 结构。观察到 3D 纳米结构的有效细胞内化，并且我们验证了可生物降解纳米结构的细胞内降解和解体。这项研究说明了可生物降解的交叉-β SPN 的潜力，并提供了一个可与自组装肽一起使用的有价值的工具包。
