Shi Yejiao, Hu Yang, Ochbaum Guy, Lin Ran, Bitton Ronit, Cui Honggang, Azevedo Helena S
School of Engineering and Materials Science, Institute of Bioengineering, Queen Mary, University of London, London, E1 4NS, UK.
Department of Chemical and Biomolecular Engineering and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Chem Commun (Camb). 2017 Jul 4;53(52):7037-7040. doi: 10.1039/c7cc03512h. Epub 2017 Jun 14.
We report here a proof-of-concept design of a multi-domain cell-penetrating peptide amphiphile (CPPA) which can self-assemble into fibrous nanostructures and transform into spherical micelles upon enzymatic degradation by matrix metalloproteinase-2 (MMP-2) up-regulated in the tumour environment. Concomitant with this morphological transition, the cell-penetrating peptide (CPP), which was previously buried inside the CPPA fibers, could be presented on the surface of the CPPA micelles, enhancing their cell-penetrating ability. These multifunctional and enzyme-responsive CPP nanostructures hold potential as nanocarriers for tumour-targeted intracellular delivery of therapeutic and diagnostic agents.
我们在此报告一种多结构域细胞穿透肽两亲分子（CPPA）的概念验证设计，该分子可自组装成纤维状纳米结构，并在肿瘤环境中上调的基质金属蛋白酶-2（MMP-2）酶解作用下转变为球形胶束。伴随着这种形态转变，先前埋在CPPA纤维内部的细胞穿透肽（CPP）可呈现在CPPA胶束表面，增强其细胞穿透能力。这些多功能且酶响应性的CPP纳米结构作为用于肿瘤靶向细胞内递送治疗和诊断剂的纳米载体具有潜力。
