Plackett P, Corner L A, Fifis T, Radford A J, Ripper J L, Chen N C, Liu Y M
C.S.I.R.O. Animal Health Research Laboratory, Parkville, Vic, Australia.
Vet Microbiol. 1989 Aug;20(4):339-48. doi: 10.1016/0378-1135(89)90058-8.
When preparations containing smooth Brucella abortus lipopolysaccharide (LPS) were used as antigen in an ELISA, strong positive reactions were obtained with sera from sheep infected with Brucella melitensis or with Brucella ovis. Oxidation of the LPS with sodium metaperiodate greatly reduced the extent of the cross-reactions with antisera to B. ovis, with little effect on the reactions with antisera to smooth B. melitensis. Periodate oxidation of hot saline extract (HSX) antigen of B. ovis markedly reduced its reactivity in ELISA with anti-B. ovis sera and eliminated cross-reactivity with anti-B. melitensis sera. The reactivity of HSX was maintained after treatment with proteinase K. A simple ELISA system, in which replicate samples from a single serum dilution were tested in parallel against both B. ovis HSX antigen and periodate-oxidised smooth phase B. abortus LPS, was evaluated. It was found to discriminate well between antibodies induced by vaccination or virulent infection with B. melitensis strains and those induced by infection with B. ovis.
当含有光滑型流产布鲁氏菌脂多糖（LPS）的制剂在酶联免疫吸附测定（ELISA）中用作抗原时，感染了羊种布鲁氏菌或绵羊布鲁氏菌的绵羊血清产生了强阳性反应。用偏高碘酸钠氧化LPS大大降低了与绵羊布鲁氏菌抗血清的交叉反应程度，而对与光滑型羊种布鲁氏菌抗血清的反应影响很小。绵羊布鲁氏菌热盐水提取物（HSX）抗原经高碘酸盐氧化后，其在ELISA中与绵羊布鲁氏菌抗血清的反应性显著降低，并消除了与羊种布鲁氏菌抗血清的交叉反应。用蛋白酶K处理后，HSX的反应性得以维持。评估了一种简单的ELISA系统，在该系统中，将来自单一血清稀释度的重复样品同时与绵羊布鲁氏菌HSX抗原和经高碘酸盐氧化的光滑型流产布鲁氏菌LPS进行平行检测。结果发现，该系统能很好地区分由羊种布鲁氏菌菌株疫苗接种或强毒感染诱导产生的抗体与由绵羊布鲁氏菌感染诱导产生的抗体。
