Malutan Andrei Mihai, Drugan Cristina, Walch Katharina, Drugan Tudor, Ciortea Razvan, Mihu Dan
Second Obstetrics and Gynecology Department, "Iuliu Hatieganu" University of Medicine and Pharmacy, 6 Louis Pasteur st, 400012, Cluj-Napoca, Cluj, Romania.
Medical Biochemistry Department, "Iuliu Hatieganu" University of Medicine and Pharmacy, 6 Louis Pasteur st, 400012, Cluj-Napoca, Cluj, Romania.
Arch Gynecol Obstet. 2017 Feb;295(2):503-510. doi: 10.1007/s00404-016-4269-5. Epub 2016 Dec 21.
Endometriosis has an incidence reaching up to 50% in infertile women. Cytokine-mediated immune responses seem to play an important role in endometriosis pathogenesis, but still the etiology and pathophysiology remain unclear. In the current study we tried to investigate whether there is a relationship between IL-10 genetic polymorphism, serum levels of IL-10 and the presence of advanced endometriosis.
The presence of IL-10 592C/A, 819T/C, 1082G/A promoter polymorphisms and IL-10 serum levels were investigated in advanced endometriosis patients compared to healthy controls. Genomic DNA was extracted from peripheral blood leukocytes and further analyzed by PCR.
IL-10 serum levels were higher in endometriosis group compared to controls (1.48, 0.68, p < 0.001). We have observed an association between IL-10 592C/C and 819C/C genotypes, presence of C alleles and an increased risk of endometriosis. No difference was observed in IL-10 serum levels corresponding to different alleles or genotypes.
Our results suggest that IL-10 592A/C and 819T/C promoter polymorphisms confer susceptibility to advanced endometriosis. No associations were found between the IL-10 1082A/G polymorphism and susceptibility to advanced endometriosis.
子宫内膜异位症在不孕女性中的发病率高达50%。细胞因子介导的免疫反应似乎在子宫内膜异位症的发病机制中起重要作用，但病因和病理生理学仍不清楚。在本研究中，我们试图探讨白细胞介素-10（IL-10）基因多态性、血清IL-10水平与重度子宫内膜异位症之间是否存在关联。
与健康对照相比，研究重度子宫内膜异位症患者中IL-10 592C/A、819T/C、1082G/A启动子多态性及血清IL-10水平。从外周血白细胞中提取基因组DNA，并通过聚合酶链反应（PCR）进一步分析。
与对照组相比，子宫内膜异位症组的血清IL-10水平更高（1.48对0.68，p<0.001）。我们观察到IL-10 592C/C和819C/C基因型、C等位基因的存在与子宫内膜异位症风险增加之间存在关联。不同等位基因或基因型对应的血清IL-10水平未观察到差异。
我们的结果表明，IL-10 592A/C和819T/C启动子多态性赋予了对重度子宫内膜异位症的易感性。未发现IL-10 1082A/G多态性与重度子宫内膜异位症易感性之间存在关联。
