a Analytical Chemistry Laboratory, Department of Chemical Engineering , Aristotle University of Thessaloniki , Thessaloniki , Greece.
b Laboratory of Forensic Medicine & Toxicology, Department of Medicine , Aristotle University of Thessaloniki , Thessaloniki , Greece.
Crit Rev Anal Chem. 2017 Jul 4;47(4):279-297. doi: 10.1080/10408347.2016.1273754. Epub 2016 Dec 22.
Screening and quantification of phthalate metabolites in biological matrices provide information on the phthalate exposure. The preferred tool for the determination of phthalate metabolites is liquid chromatography-mass spectrometry, typically preceded by a sample extraction step. Method development for the determination of phthalate metabolites by hyphenated techniques faces challenges due to the widespread occurrence of phthalates in the laboratory and sample collection materials that impairs their accurate quantification. Here, the analytical methods that have been developed for the determination of biomarkers of phthalates in various matrices are presented, and limitations and challenges in these applications are discussed.
在生物基质中筛选和定量测定邻苯二甲酸酯代谢物可提供有关邻苯二甲酸酯暴露的信息。测定邻苯二甲酸酯代谢物的首选工具是液相色谱-质谱联用技术，通常在样品提取步骤之前进行。由于邻苯二甲酸酯在实验室和样品采集材料中广泛存在，会干扰其准确定量，因此采用联用技术测定邻苯二甲酸酯代谢物的方法开发面临挑战。本文介绍了各种基质中测定邻苯二甲酸酯生物标志物的分析方法，并讨论了这些应用中的局限性和挑战。
