Division of Neuroscience, Department of Pharmacology, Shrimati Kishoritai Bhoyar College of Pharmacy, New Kamptee, Nagpur, (Maharashtra), 441 002, India, India.
Division of Neuroscience, Department of Pharmacology, Shrimati Kishoritai Bhoyar College of Pharmacy, New Kamptee, Nagpur, (Maharashtra), 441 002, India, India; Government Colleges of Pharmacy, Kathora Naka, Amravati 444604, Maharashtra, India.
Biomed Pharmacother. 2017 Feb;86:271-278. doi: 10.1016/j.biopha.2016.12.039. Epub 2016 Dec 19.
The present study investigated the pharmacological effect of agmatine in Complete Freud Adjuvant (CFA) induced arthritis and cachexia in rats. The rats were injected with CFA (0.1ml/rat) to induced symptoms of arthritis. Day 8 onwards of CFA administration, rats were injected daily with agmatine for next 7days, and arthritis score, body weights and food intake were monitored daily (g). Since cachexia is known to produce severe inflammation, malnutrition and inhibition of albumin gene expression, we have also monitored the total proteins, albumin, TNF-α and IL-6 levels in arthritic rats and its modulation by agmatine. In the present study, CFA treated rats showed a progressive reduction in both food intake and body weight. In addition analysis of blood serum of arthritis animals showed a significant reduction in proteins and albumin and significant elevation in tumor necrosis factor (TNF)-α and Interleukins (IL)-6. Chronic agmatine (20-40mg/kg, ip) treatment not only attenuated the signs of arthritis but also reverses anorexia and body weight loss in CFA treated rats. In addition, agmatine restored total protein and albumin and reduces TNF-α and IL-6 levels in arthritis rats. These results suggest that agmatine administration can prevent the body weights loss and symptoms of arthritis via inhibition of inflammatory cytokines.
本研究探讨了胍丁胺在完全弗氏佐剂（CFA）诱导的关节炎和大鼠恶病质中的药理学作用。将 CFA（0.1ml/大鼠）注射到大鼠体内以诱导关节炎症状。CFA 给药第 8 天起，大鼠每天注射胍丁胺 7 天，每天监测关节炎评分、体重和食物摄入量（g）。由于恶病质已知会产生严重的炎症、营养不良和白蛋白基因表达抑制，我们还监测了关节炎大鼠的总蛋白、白蛋白、TNF-α 和 IL-6 水平及其胍丁胺的调节作用。在本研究中，CFA 处理的大鼠表现出食物摄入量和体重的逐渐减少。此外，关节炎动物血清分析表明蛋白质和白蛋白显著减少，肿瘤坏死因子（TNF）-α 和白细胞介素（IL）-6 显著升高。慢性胍丁胺（20-40mg/kg，ip）治疗不仅减轻了关节炎的症状，还逆转了 CFA 处理大鼠的厌食和体重减轻。此外，胍丁胺还可恢复关节炎大鼠的总蛋白和白蛋白，并降低 TNF-α 和 IL-6 水平。这些结果表明，胍丁胺的给药可以通过抑制炎症细胞因子来预防体重减轻和关节炎症状。
