Hussain Asif, Aslam Bilal, Muhammad Faqir, Faisal Muhammad Naeem, Kousar Shaneel, Mushtaq Aqsa, Bari Muhammad Usman
Institute of Physiology and Pharmacology, University of Agriculture, Faisalabad-38040, Faisalabad, Punjab, Pakistan.
Iran J Basic Med Sci. 2021 Jul;24(7):951-961. doi: 10.22038/ijbms.2021.55145.12355.
This study aimed to evaluate the anti-arthritic activity of leaves' and roots' hydroalcoholic extracts in Complete Freund's adjuvant-induced arthritis in Wistar rats.
HPLC and FT-IR analysis detected pharmacologically important phytocompounds in both plant extracts. Oral treatments including methotrexate (MTX; 3 mg/kg twice a week) and extracts at 250 and 500 mg/kg/day were initiated after arthritis induction. Changes in paw swelling, arthritic score, body weight, organ indices (thymus and spleen), hematological and biochemical parameters, and pro-/anti-inflammatory cytokine expression using qRT-PCR were assessed. Oxidative stress markers in hepatic tissue were determined. Histopathological and radiological examinations were also performed.
RCE ( extract) and WSE ( extract) demonstrated a reduction in paw swelling, arthritic score, and restoration of body weight and organ indices. Hematological parameters, serum inflammatory markers such as CRP and RF, and liver function markers of arthritic rats were significantly (<0.01) ameliorated with RCE and WSE treatment. Both plants persuasively down-regulated IL-1β, IL-6, IL-17a, TNF-α, and RANKL and up-regulated IL-4, INF-γ, and OPG relative expression as well as alleviating hepatic oxidative stress parameters. Histopathological and radiological findings revealed a marked reduction in tissue inflammation and bone erosion in extracts treated groups.
The study findings suggest that leaves and roots have markedly subsided inflammation and improved health through modulating pro-/anti-inflammatory cytokine expression and reducing oxidative stress.
本研究旨在评估叶和根的水醇提取物对弗氏完全佐剂诱导的Wistar大鼠关节炎的抗关节炎活性。
高效液相色谱（HPLC）和傅里叶变换红外光谱（FT-IR）分析检测了两种植物提取物中具有药理重要性的植物化合物。在诱导关节炎后开始口服治疗，包括甲氨蝶呤（MTX；3mg/kg，每周两次）以及250和500mg/kg/天的提取物。评估爪肿胀、关节炎评分、体重、器官指数（胸腺和脾脏）、血液学和生化参数的变化，以及使用定量逆转录聚合酶链反应（qRT-PCR）检测促炎/抗炎细胞因子的表达。测定肝组织中的氧化应激标志物。还进行了组织病理学和放射学检查。
RCE（叶提取物）和WSE（根提取物）表现出爪肿胀减轻、关节炎评分降低，体重和器官指数恢复。RCE和WSE治疗显著（<0.01）改善了关节炎大鼠的血液学参数、血清炎症标志物如C反应蛋白（CRP）和类风湿因子（RF）以及肝功能标志物。两种植物均有说服力地下调白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-17a（IL-17a）、肿瘤坏死因子-α（TNF-α）和核因子κB受体活化因子配体（RANKL），上调白细胞介素-4（IL-4）、干扰素-γ（INF-γ）和骨保护素（OPG）的相对表达，并减轻肝脏氧化应激参数。组织病理学和放射学结果显示，提取物治疗组的组织炎症和骨质侵蚀明显减轻。
研究结果表明，叶和根通过调节促炎/抗炎细胞因子表达和降低氧化应激，显著减轻了炎症并改善了健康状况。
