Seifi Safoura, Seyedmajidi Maryam, Salehinejad Jahanshah, Gholinia Hemmat, Aliakbarpour Fatemeh
Department of Oral and Maxillofacial Pathology, School of Dentistry, Babol University of Medical Sciences, Babol, Iran.
Dental Research Centre, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Otorhinolaryngol. 2016 Nov;28(89):389-397.
Natural killer (NK) cells, of which CD is a specific marker, play an important role in host defense against tumors. Cancer stem cells, of which aldehyde dehydrogenase isoform 1 (ALDH) is an immunohistochemical marker, are a group of tumorigenic cells which are involved in migration and tumor recurrences. We aimed to evaluate the expression of ALDH and CD in common salivary gland tumors, as well as their relationship with each other and with a number of clinicopathologic factors.
Forty-five paraffin blocks of salivary gland tumors (pleomorphic adenoma, mucoepidermoid carcinoma and adenoid cystic carcinoma, 15 samples each) were selected. Malignant tumors were classified into two groups: low-grade (including mucoepidermoid carcinoma grade I) and high-grade (including mucoepidermoid carcinoma grade III and adenoid cystic carcinoma). Immunohistochemical staining for ALDH and CD markers was performed. Data were analyzed using SPSS (20) and the Chi-square test.
CD expression was significantly higher in benign and high-grade malignant tumors (P=0.01). ALDH overexpressed in all three salivary tumors, but not to statistically significant degree (P=0.54). There was no statistically significant correlation between ALDH and CD expression with demographic factors (age, gender, or location of tumor; P>0.05).
It appears that the number of NK cells and their function change in different types of salivary gland tumors (benign/malignant) and stroma. NK cells are important components of the anti-tumor system; therefore immune dysfunction is associated with tumor progression in tumors of the salivary gland. ALDH overexpression suggests its role in tumorogenesis, but ALDH is not involved in the morphogenesis of salivary gland tumors.
自然杀伤（NK）细胞以CD为特异性标志物，在宿主抗肿瘤防御中发挥重要作用。癌症干细胞以醛脱氢酶同工酶1（ALDH）作为免疫组化标志物，是一组参与迁移和肿瘤复发的致瘤细胞。我们旨在评估ALDH和CD在常见涎腺肿瘤中的表达情况，以及它们之间的相互关系和与一些临床病理因素的关系。
选取45例涎腺肿瘤石蜡块（多形性腺瘤、黏液表皮样癌和腺样囊性癌各15例）。恶性肿瘤分为两组：低级别（包括Ⅰ级黏液表皮样癌）和高级别（包括Ⅲ级黏液表皮样癌和腺样囊性癌）。对ALDH和CD标志物进行免疫组化染色。使用SPSS（20）软件和卡方检验分析数据。
CD在良性和高级别恶性肿瘤中的表达显著更高（P = 0.01）。ALDH在所有三种涎腺肿瘤中均有过表达，但差异无统计学意义（P = 0.54）。ALDH和CD的表达与人口统计学因素（年龄、性别或肿瘤位置）之间无统计学显著相关性（P>0.05）。
不同类型的涎腺肿瘤（良性/恶性）及间质中NK细胞的数量及其功能似乎发生了变化。NK细胞是抗肿瘤系统的重要组成部分；因此，免疫功能障碍与涎腺肿瘤的进展相关。ALDH过表达提示其在肿瘤发生中的作用，但ALDH不参与涎腺肿瘤的形态发生。
